{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:55:59Z","timestamp":1760061359306,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Quantification of cyanobacterial CO<jats:sub>2<\/jats:sub> fixation rates is vital to determining their potential as industrial strains in a circular bioeconomy. Currently, however, CO<jats:sub>2<\/jats:sub> fixation rates are most often determined through indirect and\/or low\u2010resolution methods, resulting in an incomplete picture of both dynamic behaviors and total carbon fixation potential. To address this, we developed the \u201cAutomated Carbon and CO<jats:sub>2<\/jats:sub> Experimental Sampling System\u201d (ACCESS); a low\u2010cost system for in situ off\u2010gas analysis that supports the automated acquisition of high\u2010resolution volumetric CO<jats:sub>2<\/jats:sub> uptake rates from multiple cyanobacterial cultures in parallel. Carbon fixation data obtained via ACCESS were first independently validated by elemental analysis of cultivated biomass. Using ACCESS, we then demonstrate how the volumetric CO<jats:sub>2<\/jats:sub> uptake rate of two model cyanobacteria, <jats:italic>Synechococcus sp<\/jats:italic>. PCC 7002 and <jats:italic>Synechocystis sp<\/jats:italic>. PCC 6803, accelerates linearly to a maximum before then decaying monotonically to cessation by stationary phase. Furthermore, consistent with the expected stoichiometry, strong correlations were also found to exist between cell growth and carbon fixation, both in terms of rates as well as total levels. The novel insights made possible via ACCESS will aid other cyanobacterial researchers in diverse fundamental and applied research efforts.<\/jats:p>","DOI":"10.1002\/bit.28905","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:39:17Z","timestamp":1734568757000},"page":"594-605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Situ, High\u2010Resolution Quantification of CO<sub>2<\/sub> Uptake Rates via Automated Off\u2010Gas Analysis Illuminates Carbon Uptake Dynamics in Cyanobacterial Cultures"],"prefix":"10.1002","volume":"122","author":[{"given":"Christopher M.","family":"Jones","sequence":"first","affiliation":[{"name":"School for Engineering of Matter, Transport and Energy Arizona State University Tempe Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean","family":"Innes","sequence":"additional","affiliation":[{"name":"School for 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