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This study reports the first systematic engineering of\n                    <jats:italic>C. tyrobutyricum<\/jats:italic>\n                    for the heterologous production of 1,3\u2010propanediol (1,3\u2010PDO) from industrial by\u2010product crude glycerol. Initially, the glycerol reductive pathway for 1,3\u2010PDO production was constructed, and the unique glycerol oxidation pathway in\n                    <jats:italic>C. tyrobutyricum<\/jats:italic>\n                    was elucidated. Subsequently, the glycerol metabolism and 1,3\u2010PDO synthesis pathways were enhanced. Furthermore, the intracellular reducing power supply and the fermentation process were optimized to improve 1,3\u2010PDO production. Consequently, 54.06\u2009g\/L 1,3\u2010PDO with a yield of 0.64\u2009mol\/mol and a productivity of 1.13\u2009g\/L\u00b7h was obtained using crude glycerol and fish meal. The strategies described herein could facilitate the engineering of\n                    <jats:italic>C. tyrobutyricum<\/jats:italic>\n                    as a robust host for synthesizing valuable chemicals.\n                  <\/jats:p>","DOI":"10.1002\/bit.29010","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T02:00:36Z","timestamp":1745200836000},"page":"1804-1815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Systems Metabolic Engineering of\n                    <i>Clostridium tyrobutyricum<\/i>\n                    for 1,3\u2010Propanediol Production From Crude Glycerol"],"prefix":"10.1002","volume":"122","author":[{"given":"Yongzhang","family":"Mo","sequence":"first","affiliation":[{"name":"School of Biology and Biological Engineering South China University of Technology Guangzhou 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