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Currently, the composition of cell types generated within these SC\u2010islets cannot be controlled via soluble factors during this differentiation process and consist of off\u2010target cell types. In this study, we devised a magnetic\u2010activated cell sorting protocol to enrich SC\u2010islets for CD49a, a marker associated with functional insulin\u2010producing \u03b2 cells. SC\u2010islets were generated from human pluripotent stem cells using an adherent differentiation protocol and then sorted and aggregated into islet\u2010like clusters to produce CD49a\u2010enriched, CD49a\u2010depleted, and unsorted SC\u2010islets. Single\u2010cell RNA sequencing (scRNA\u2010seq) and immunostaining revealed that CD49a\u2010enriched SC\u2010islets had higher proportions of \u03b2 cells and improved transcriptional identity compared to other cell types. Functional assays demonstrated that CD49a\u2010enriched SC\u2010islets exhibited enhanced glucose\u2010stimulated insulin secretion both In vitro and In Vivo following transplantation into diabetic mice. These findings suggest that CD49a\u2010based sorting significantly improves \u03b2 cell identity and the overall function of SC\u2010islets, improving their effectiveness for T1D cell replacement therapies.<\/jats:p>","DOI":"10.1002\/bit.29030","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T01:39:12Z","timestamp":1747877952000},"page":"2206-2217","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Controlling Human Stem Cell\u2010Derived Islet Composition Using Magnetic Sorting"],"prefix":"10.1002","volume":"122","author":[{"given":"Allison B.","family":"Kelley","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Metabolism and Lipid Research Washington University School of Medicine St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mira","family":"Shunkarova","sequence":"additional","affiliation":[{"name":"Division of 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