{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:16:51Z","timestamp":1785248211982,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T00:00:00Z","timestamp":1751673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The rise in cancer, autoimmune, inflammatory, and infectious diseases in recent decades has led to a surge in the development of monoclonal antibodies (mAbs) therapies, now the most widely used family of biologics. To meet the growing global demand, biopharmaceutical industries are intensifying their production processes. One approach to achieve more efficient production of effective mAbs is to develop tools for real\u2010time quality monitoring. Specifically, the glycosylation profile of mAbs must be closely monitored, since it greatly impacts their therapeutic efficacy and innocuity, making it a critical quality attribute. In this study, we developed a surface plasmon resonance\u2010based integrated assay allowing for the simultaneous quantification and glycosylation characterization of mAbs in crude samples, hence permitting the at\u2010line analysis of bioreactor cell cultures. Thanks to the high specificity of the interaction between biosensor surface\u2010bound protein A and the Fc region of mAbs, we quantified crude IgG samples under mass transport limitations. Next, by flowing running buffer on the surface, impurities contained in the mAbs samples were washed away from the biosensor surface, allowing subsequent recording of the kinetics between the captured mAbs and injected Fc\u03b3RII receptors. Of interest, with this strategy, we were able to quantify terminal galactosylation and core fucosylation of IgG lots, two important glycan modifications for mAb efficacy.<\/jats:p>","DOI":"10.1002\/bit.70016","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T07:34:07Z","timestamp":1751700847000},"page":"2724-2738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Surface Plasmon Resonance\u2010Based Integrated Assay for Quantification and Glycosylation Characterization of Monoclonal Antibodies in Crude Heterogeneous Samples"],"prefix":"10.1002","volume":"122","author":[{"given":"Ilona","family":"Metayer","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Polytechnique Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Forest\u2010Nault","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Polytechnique Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Guimond","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics Research Centre National Research Council Canada Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0405-8946","authenticated-orcid":false,"given":"Simon","family":"Joubert","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics Research Centre National Research Council Canada Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2106-1331","authenticated-orcid":false,"given":"Olivier","family":"Henry","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Polytechnique Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2268-4111","authenticated-orcid":false,"given":"Yves","family":"Durocher","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics Research Centre National Research Council Canada Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6280-1570","authenticated-orcid":false,"given":"Gregory","family":"De Crescenzo","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Polytechnique Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy","family":"Gaudreault","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Polytechnique Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2019.1608143"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1468"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antib9020022"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2019.1581017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crimmu.2020.06.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.04.019"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.202400031"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2020.e00424"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwz065"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12195-008-0035-5"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3917\/rindu1.234.0010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b00342"},{"key":"e_1_2_10_14_1","unstructured":"Food and Drug Administration. (2004).Guidance for Industry\u2014PAT \u2010 A Framework for Innovative Pharmaceutical Development Manufacturing and Quality Assurance."},{"key":"e_1_2_10_15_1","unstructured":"Food and Drug Administration. (2009).Guidance for Industry\u2014Q8(R2)\u2014Pharmaceutical Development."},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126616"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr9111996"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28673"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1651"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13010048"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20729"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwr027"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.929895"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c05396"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-003-2101-0"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp079"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21725"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28127"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1911.11066"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.641521"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28129"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120420"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/abt\/tbac021"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.4477"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1352(199909\/10)12:5<279::AID-JMR473>3.0.CO;2-3"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.01.031"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-42925-1"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2015.1029215"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394802-1.00009-1"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.02.013"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122540"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.12.005"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2016.1218586"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.25520"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2024.2374607"},{"key":"e_1_2_10_46_1","unstructured":"WHO. (2024).Press Release n\u00b0345\u2014Global Cancer Burden Growing Amidst Mounting Need for Services.https:\/\/www.iarc.who.int\/wp-content\/uploads\/2024\/02\/pr345_E.pdf."},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20151"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2008.10.011"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty319"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:47:38Z","timestamp":1760042858000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.70016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,5]]},"references-count":48,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/bit.70016"],"URL":"https:\/\/doi.org\/10.1002\/bit.70016","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,5]]},"assertion":[{"value":"2025-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}