{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:12:53Z","timestamp":1788347573923,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Itaconic acid is a monomer for high performance polymers. While produced in industry with the filamentous fungi\n                    <jats:italic>Aspergillus terreus<\/jats:italic>\n                    , the production with the smut fungus\n                    <jats:italic>Ustilago maydis<\/jats:italic>\n                    was proposed recently. The strict aerobic process suffers from high power input via gassing and stirring. Here, we investigated in detail possible scenarios for the reduction of energy use during cultivation. In contrast to fermentations with other organic acids, in which even small oxygen concentrations in the medium hinders production, low oxygen availability correlated with increased itaconic acid titer and yield. This is somewhat surprising, as not only the sensitivity of itaconic acid producing\n                    <jats:italic>U. maydis<\/jats:italic>\n                    to oxygen deprivation was previously reported, but also the lower degree of reduction compared to glucose is not directly arguing for improved yield. Scale ups from 0.4 to 30\u2009L using different criteria confirmed the positive impact of low availability of oxygen on itaconic acid production, suggesting a shift in metabolic pathways under restricted oxygen conditions. Oxygen limitation, encountered more likely in industrial fermenters, can be effectively used as a process control strategy to enhance itaconic acid production in\n                    <jats:italic>U. maydis<\/jats:italic>\n                    , offering a new approach for improving the efficiency of industrial\u2010scale biotechnological production.\n                  <\/jats:p>","DOI":"10.1002\/bit.70035","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T07:55:27Z","timestamp":1754121327000},"page":"3007-3017","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Low Oxygen Availability Increases Itaconate Production by\n                    <i>Ustilago maydis<\/i>"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1091-4324","authenticated-orcid":false,"given":"Marianne","family":"Volkmar","sequence":"first","affiliation":[{"name":"Institute of Bioprocess Engineering University of Kaiserslautern\u2011Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Laudensack","sequence":"additional","affiliation":[{"name":"Institute of Bioprocess Engineering University of Kaiserslautern\u2011Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Bartzack","sequence":"additional","affiliation":[{"name":"Institute of Bioprocess Engineering University of Kaiserslautern\u2011Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Erdmann","sequence":"additional","affiliation":[{"name":"Institute of Bioprocess Engineering University of Kaiserslautern\u2011Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Sch\u00f6nrock","sequence":"additional","affiliation":[{"name":"Karlsruhe Institute of Technology Institute of Process Engineering in Life Sciences Karlsruhe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emely","family":"Fuderer","sequence":"additional","affiliation":[{"name":"Karlsruhe Institute of Technology Institute of Process Engineering in Life Sciences Karlsruhe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Holtmann","sequence":"additional","affiliation":[{"name":"Karlsruhe Institute of Technology Institute of Process Engineering in Life Sciences Karlsruhe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars M.","family":"Blank","sequence":"additional","affiliation":[{"name":"ABBt \u2013 Aachen Biology and Biotechnology, iAMB \u2010 Institute of Applied Microbiology RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7674-0967","authenticated-orcid":false,"given":"Roland","family":"Ulber","sequence":"additional","affiliation":[{"name":"Institute of Bioprocess Engineering University of Kaiserslautern\u2011Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-017-1388-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2017.1312268"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13525"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7010020"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/fluids9050115"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-6-1395"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2008.05.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi052045o"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-49068-5"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00436063"},{"key":"e_1_2_9_12_1","volume-title":"Bioprocess Engineering Principles","author":"Doran P. 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