{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:07:42Z","timestamp":1792019262233,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Here, we employed human embryonic stem cell spheroid (3D hESCs)\u2010derived exosomes to assess inflammatory responses of macrophages in liver fibrosis, and found that 3D hESC\u2010exosomes promoted the transformation of macrophages from M1 to M2 phenotype in vitro. The transplantation of 3D hESC\u2010exosomes exhibited a notable decrease of pro\u2010inflammatory factors, and significant increase of anti\u2010inflammatory factors, and led to a reduction of CD86\n                    <jats:sup>+<\/jats:sup>\n                    M1 macrophages and an increase of Arg\u20101\n                    <jats:sup>+<\/jats:sup>\n                    M2 macrophages in the livers of treated fibrotic mice, restricting the advancement of liver fibrosis. hsa_circ_0076798, derived from 3D hESC\u2010exosomes, acts as a molecular sponge that sequesters miR\u20101184, thereby mitigating miR\u20101184\u2010mediated repression of DICER1 expression within macrophages. Further investigation revealed that DICER1 ultimately mitigated macrophage inflammation by deactivating TNF\/NF\u2010\u03baB signaling pathway. Therefore, our findings demonstrated that 3D hESC\u2010exosomes alleviated inflammatory responses by suppressing TNF\/NF\u2010\u03baB pathway through hsa_circ_0076798\/miR\u20101184\/DICER1 axis, and offers insights into the targeted treatment of liver fibrosis via exosome\u2010based cell\u2010free therapy.\n                  <\/jats:p>","DOI":"10.1002\/bit.70047","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T06:43:39Z","timestamp":1755585819000},"page":"3073-3089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["3D hESC Exosome\u2010Derived Circular RNA hsa_circ_0076798 Inhibits M1 Macrophage Inflammatory Response and Alleviates Liver Fibrosis by Suppressing TNF\/NF\u2010\u03baB Pathway Through miR\u20101184\/DICER1 Axis"],"prefix":"10.1002","volume":"122","author":[{"given":"Yuwei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, the Second Affiliated Hospital, School of Medicine South China University of Technology Guangzhou Guangdong Province P.R. 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