{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:59:06Z","timestamp":1787903946667,"version":"build-2784847793"},"reference-count":56,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Heyndrickxia coagulans<\/jats:italic>\n                    is the dominant strain in L\u2010lactic acid fermentation. To promote the development of green and low\u2010cost L\u2010lactic acid fermentation processes, it is crucial to understand their solid\u2010state fermentation (SSF) performance in lignocellulose raw materials. This study presents the first whole genome analysis of\n                    <jats:italic>H. coagulans L\u20102<\/jats:italic>\n                    (single chromosome, 3.58\u2009Mb, 46.28% GC content) to evaluate its SSF potential using sweet sorghum stalk. COG and KEGG annotations revealed that\n                    <jats:italic>H. coagulans L\u20102<\/jats:italic>\n                    metabolizes diverse hexoses and pentoses for L\u2010lactic acid production, and the key enzymatic sequences in carbohydrate metabolism exhibited high similarity with other\n                    <jats:italic>Heyndrickxia<\/jats:italic>\n                    strains. Comparative genomics with ATCC7050 demonstrated 70% genome similarity (2148 collinear CDSs), while\n                    <jats:italic>L\u20102<\/jats:italic>\n                    has a more comprehensive carbohydrate metabolism network, especially in pentose metabolism pathways, such as arabinose, mannose, and rhamnose. Through the result of batch solid fermentation,\n                    <jats:italic>H. coagulans L\u20102<\/jats:italic>\n                    could promote the re\u2010fermentation of L\u2010lactic acid in silage and reduce heterotypic lactic acid fermentation (lactic acid\/ethanol ratio increased from 3.890 to 8.103), which indicated that the strain\n                    <jats:italic>L\u20102<\/jats:italic>\n                    played a key role in the SSF of sweet sorghum silage and promoted the production of bio\u2010based materials precursors of sweet sorghum. This study provides a new strategy for the high\u2010value utilization of lignocellulosic biomass.\n                  <\/jats:p>","DOI":"10.1002\/bit.70065","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T05:22:42Z","timestamp":1757654562000},"page":"3285-3297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of Solid\u2010State Fermentation Potential of Sweet Sorghum Stalk by\n                    <i>Heyndrickxia coagulans (Bacillus coagulans) L\u20102<\/i>\n                    Based on Complete Genome and Phenotypic Analysis"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3989-047X","authenticated-orcid":false,"given":"Li","family":"Wei","sequence":"first","affiliation":[{"name":"Institute of New Energy Technology Tsinghua University Beijing China"},{"name":"State Key Laboratory of Urban Water Resource and Environment Harbin 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