{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T14:00:43Z","timestamp":1792245643051,"version":"4.4.0"},"reference-count":62,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Amid growing concerns about climate change, environmental pollution, and fossil resource depletion, bio\u2010based chemicals and materials have garnered significant attention. Succinic acid (SA), a four\u2010carbon dicarboxylic acid, is a key platform chemical for producing industrially important compounds such as poly(butylene succinate) (PBS), a biodegradable polymer. In this paper, we demonstrate the enhanced production of SA using metabolically engineered\n                    <jats:italic>Mannheimia succiniciproducens<\/jats:italic>\n                    PALK (pMS3\u2010gltA) strain, which achieves high SA yields through enhanced biomass production and optimized metabolic pathways. High\u2010purity SA, purified from the fermentation broth, was used in an environmentally friendly two\u2010step polymerization process under mild conditions. In the first step, diethyl succinate was synthesized from SA and ethanol using Amberlyst as a catalyst. This was followed by polymerization with 1,4\u2010butanediol, catalyzed by\n                    <jats:italic>Candida antarctica<\/jats:italic>\n                    lipase B. The resulting PBS exhibited comparable thermal stability and molecular weight distribution to its petrochemical counterpart, with enhanced biodegradability. By integrating metabolic engineering with sustainable polymer synthesis, this study underscores the potential for an eco\u2010friendly approach to developing bio\u2010based polymers, offering a promising solution to the environmental challenges posed by conventional plastics.\n                  <\/jats:p>","DOI":"10.1002\/bit.70072","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T11:02:26Z","timestamp":1758711746000},"page":"3393-3405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Succinic Acid Production by Engineered\n                    <i>Mannheimia succiniciproducens<\/i>\n                    and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis"],"prefix":"10.1002","volume":"122","author":[{"given":"Ji Yeon","family":"Kim","sequence":"first","affiliation":[{"name":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea"},{"name":"Systems Metabolic Engineering and Systems Healthcare Cross\u2010Generation Collaborative Laboratory KAIST Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong An","family":"Lee","sequence":"additional","affiliation":[{"name":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea"},{"name":"Systems Metabolic Engineering and Systems Healthcare Cross\u2010Generation Collaborative Laboratory KAIST Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gi Bae","family":"Kim","sequence":"additional","affiliation":[{"name":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea"},{"name":"Systems Metabolic Engineering and Systems Healthcare Cross\u2010Generation Collaborative Laboratory KAIST Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngjoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea"},{"name":"Systems Metabolic Engineering and Systems Healthcare Cross\u2010Generation Collaborative Laboratory KAIST Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0599-3091","authenticated-orcid":false,"given":"Sang Yup","family":"Lee","sequence":"additional","affiliation":[{"name":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea"},{"name":"Systems Metabolic Engineering and Systems Healthcare Cross\u2010Generation Collaborative Laboratory KAIST Daejeon Republic of Korea"},{"name":"KAIST Institute for the BioCentury KAIST Daejeon Republic of Korea"},{"name":"BioProcess Engineering Research Center KAIST Daejeon Republic of Korea"},{"name":"Graduate School of Engineering Biology KAIST Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2009.11.042"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-013-1269-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-018-2016-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15839-z"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"155","article-title":"Comparative Biodegradational Analysis of Poly(Butylene Succinate) Using Pseudomonas fluorescens and Bacillus Species","volume":"1","author":"Asheeba S. 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