{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T11:52:10Z","timestamp":1774007530504,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>As viral diseases like influenza, COVID\u201019, and respiratory syncytial virus (RSV) become prevalent, demand for treatments and vaccinations is on the rise. Despite widespread vaccination, concerns remain that emerging virus strains may evade immunity. Monitoring these pathogens is crucial for developing effective treatments, but large\u2010scale population screening, while a public health need, is challenging in terms of speed, efficiency, and cost. Herein we demonstrate the use of a high\u2010throughput, chip\u2010based platform that is scalable to population\u2010wide immune surveillance using SARS\u2010CoV\u20102 pseudovirus surrogates. We tested antibodies against pseudovirus surrogates bearing the spike protein of the Wuhan\u2010Hu\u20101 (WT) SARS\u2010CoV\u20102 on\u2010chip and screened patient\u2010derived sera and eluates from patient\u2010derived and contrived Dried Blood Spots (cDBS) for effectiveness against the WT strain. Interestingly, some sera and cDBS eluates inhibited infection by the Omicron BA.4\/5 variant, which emerged a year after sample collection, suggesting cross\u2010reactivity against distinct SARS\u2010CoV\u20102 spike proteins. This chip\u2010based platform may be used to screen populations for existing immunity and immune escape of emerging viruses and their variants from prior infection and\/or vaccination.<\/jats:p>","DOI":"10.1002\/bit.70143","type":"journal-article","created":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T07:15:19Z","timestamp":1766646919000},"page":"876-885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Chip\u2010Based Surveillance Platform for Detecting Antibody\u2010Based Immunity and Immune Escape Using SARS\u2010CoV\u20102 Pseudovirus Surrogates"],"prefix":"10.1002","volume":"123","author":[{"given":"Maisha M.","family":"Feroz","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering Rensselaer Polytechnic Institute Troy New York USA"},{"name":"Center for Biotechnology &amp; Interdisciplinary Studies Rensselaer Polytechnic Institute Troy New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok\u2010Joon","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Rensselaer Polytechnic Institute Troy New York USA"},{"name":"Center for Biotechnology &amp; Interdisciplinary Studies Rensselaer Polytechnic Institute Troy New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherin","family":"Berman","sequence":"additional","affiliation":[{"name":"New York State Department of Health Division of Infectious Diseases, Wadsworth Center Albany New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Mantis","sequence":"additional","affiliation":[{"name":"New York State Department of Health Division of Infectious Diseases, Wadsworth Center Albany New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7802-3702","authenticated-orcid":false,"given":"Jonathan S.","family":"Dordick","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Rensselaer Polytechnic Institute Troy New York USA"},{"name":"Center for Biotechnology &amp; Interdisciplinary Studies Rensselaer Polytechnic Institute Troy New York USA"},{"name":"Department of Biological Sciences Rensselaer Polytechnic Institute Troy New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1033","article-title":"A Serological Assay to Detect SARS\u2010CoV\u20102 Seroconversion in Humans","volume":"26","author":"Amanat F.","year":"2020","journal-title":"Nature Medicine (New York, NY, United States)"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00846-24"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyaa198"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1184362"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2017.006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2021.108918"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofaf123"},{"key":"e_1_2_8_10_1","article-title":"Dried Blood Spots \u2010 Preparing and Processing for Use in Immunoassays and in Molecular Techniques","volume":"97","author":"Gr\u00fcner N.","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012383"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.052"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45404-x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12091011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13875"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104572"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43856-021-00045-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.22"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4739"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2020.05.008"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106648"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.146316"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2020.114025"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd3871"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fdata.2020.565589"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17892-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe1107"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.10.001"},{"key":"e_1_2_8_29_1","first-page":"58","article-title":"Comparison Study of the Bio\u2010Plex and Meso Scale Multiplexed SARS\u2010CoV\u20102 Serology Assays Reveals Evidence of Diminished Host Antibody Responses to SARS\u2010CoV\u20102 After Monoclonal Antibody Treatment","volume":"9","author":"Parikh U. 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