{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:32:11Z","timestamp":1786977131088,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Peptide chemical modification is a valuable technique for improving peptide stability and bioactivity, particularly in drug discovery applications. Here, we report the development of a novel linker junction strategy using strain\u2010promoted azide\u2010alkyne cycloaddition (SPAAC) that enables the efficient formation of branched puromycin linkers with an average yield of 97%. This approach represents an improvement over traditional Michael\u2010Addition methods, which typically yield ~15%\u201320%. The high yield of the SPAAC reaction and the near absence of by\u2010products make the linkage by click reaction easy to purify. We demonstrate the utility of our linker design by successfully performing cDNA display and chemical modification strategies such as bicyclization of peptides. Our study demonstrates the functionality of the cDNA display system with the newly incorporated junction. In addition, the successful introduction of peptide bicyclization via tris\u2010bromomethyl\u2010benzene (TBMB) in cDNA display serves as a proof\u2010of\u2010concept for complex chemical modifications. Furthermore, the position of puromycin, which disrupts protein biosynthesis, has been determined. This approach offers novel insights into the discovery of chemically modified peptides and has the potential to accelerate the development of peptide\u2010based therapeutics and diagnostics.<\/jats:p>","DOI":"10.1002\/bit.70164","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T09:40:09Z","timestamp":1769679609000},"page":"1399-1404","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High Yield Branched Puromycin Linker Design Enables Efficient cDNA Display and Chemical Modification of Peptides"],"prefix":"10.1002","volume":"123","author":[{"given":"Simon","family":"Schneider","sequence":"first","affiliation":[{"name":"Department of Nuclear Medicine, University Medical Center Freiburg, Faculty of Medicine University of Freiburg Freiburg Germany"},{"name":"Division of Radiopharmaceutical Development, German Cancer Consortium (DKTK) Partner Site Freiburg Freiburg Germany"},{"name":"German Cancer Research Center Heidelberg Germany"},{"name":"Faculty of Biology University of Freiburg Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Boll","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medical Center Freiburg, Faculty of Medicine University of Freiburg Freiburg Germany"},{"name":"Division of Radiopharmaceutical Development, German Cancer Consortium (DKTK) Partner Site Freiburg Freiburg Germany"},{"name":"German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Eder","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medical Center Freiburg, Faculty of Medicine University of Freiburg Freiburg Germany"},{"name":"Division of Radiopharmaceutical Development, German Cancer Consortium (DKTK) Partner Site Freiburg Freiburg Germany"},{"name":"German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann\u2010Christin","family":"Eder","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medical Center Freiburg, Faculty of Medicine University of Freiburg Freiburg Germany"},{"name":"Division of Radiopharmaceutical Development, German Cancer Consortium (DKTK) Partner Site Freiburg Freiburg Germany"},{"name":"German Cancer Research Center Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","unstructured":"Anand U. 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R.Maharani A. M.Gazzali andM.Muchtaridi.2022. \u201cCyclic Peptides for the Treatment of Cancers: A Review.\u201dMolecules.https:\/\/doi.org\/10.3390\/molecules271444.","DOI":"10.3390\/molecules27144428"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27696"},{"key":"e_1_2_7_18_1","unstructured":"Warnken U. M.Schn\u00f6lzer andE.Lindner.2016. WO2016120247A1. Patent."},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp514"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAD643"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22083973"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70164","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.70164","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:08:08Z","timestamp":1780326488000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.70164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,29]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/bit.70164"],"URL":"https:\/\/doi.org\/10.1002\/bit.70164","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,29]]},"assertion":[{"value":"2025-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}