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From a molecular perspective, molecular dynamics simulations have confirmed that van der Waals forces and hydrogen bond interactions in acidic environments effectively stabilized the structure\u2013activity relationship of dehydrogenase and GlcA. Leveraging this insight, multi\u2010strategies were implemented, including pH regulation, high\u2010density biocatalysis, dehydrogenase overexpression, adaptive acclimation, bioreactor scale\u2010up, and cell recycling technology, to significantly intensify the GA production. As a result, the recombinant\n                    <jats:italic>G. oxydans<\/jats:italic>\n                    achieved an effective production of 10.27\u2009g\/L GA with a productivity of 0.21\u2009g\/L\/h, representing a 366.4% intensification over the unregulated system. In comparison with the highest level of biological methods reported to date, the process demonstrates greater economic feasibility and industrial application potential. This work elucidated the molecular mechanism of acid\u2010intensified biocatalysis and provided a sustainable, industrially viable GA production platform.\n                  <\/jats:p>","DOI":"10.1002\/bit.70236","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T05:51:17Z","timestamp":1778651477000},"page":"2037-2047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating Green Process Engineering With Whole\u2010Cell Catalysis to Intensify Sustainable Glucaric Acid Production"],"prefix":"10.1002","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3688-934X","authenticated-orcid":false,"given":"Xia","family":"Hua","sequence":"first","affiliation":[{"name":"State Key Laboratory for the Development &amp; Utilization of Forest Food Resource Nanjing Forestry University Nanjing Jiangsu People's Republic of China"},{"name":"Jiangsu Co\u2010Innovation Center of 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