{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:02:50Z","timestamp":1784887370702,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Copper mining industry generates water with high sulfate (SO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    ) concentrations. The hydrogen\u2010based membrane biofilm reactor (H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010MBfR) is a promising sulfidogenesis solution, using H\n                    <jats:sub>2<\/jats:sub>\n                    as a clean electron donor. An H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010MBfR was operated for 209 days treating synthetic mining\u2010influenced water under varying conditions (HRT, H\n                    <jats:sub>2<\/jats:sub>\n                    pressure, SO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    loadings). The system proved highly resilient, achieving stable SO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    removal (&gt;\u200990%) and fully recovering from a severe shock load (4500\u2009mg S L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). To identify the governing mechanisms, a transient pseudoanalytical biofilm model was developed. A Global Sensitivity Analysis (Sobol) showed performance is dominated by the maximum specific growth rate (), detachment coefficient (), and H\n                    <jats:sub>2<\/jats:sub>\n                    S inhibition (). The model was calibrated on Stages 1\u20133 (Days 0\u2013169,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944), achieving R\u00b2\u2009=\u20090.962 (calibration) and R\u00b2\u2009=\u20090.572 (blind validation, Stage 4). Crucial findings from the model calibration (\u2009=\u20090.344\u2009d\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and a diagnostic analysis using the Thiele Modulus (mean \u2009=\u20090.224) strongly indicate that the system operated in a kinetically\u2010limited regime. Performance was governed by the delicate balance of slow growth and high detachment, not by mass transfer (H\n                    <jats:sub>2<\/jats:sub>\n                    supply or SO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    diffusion). This research validates the H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010MBfR for high\u2010rate applications and correctly shifts the bottleneck for future optimization from mass transfer to biofilm retention kinetics.\n                  <\/jats:p>","DOI":"10.1002\/bit.70246","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:05:09Z","timestamp":1779188709000},"page":"2113-2126","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Resilient High\u2010Rate Sulfidogenesis in a Hydrogen\u2010Based Membrane Biofilm Reactor: Mechanistic Analysis of Kinetic\u2010Limited Performance Under Variable Loading"],"prefix":"10.1002","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-2949","authenticated-orcid":false,"given":"Jos\u00e9","family":"Su\u00e1rez","sequence":"first","affiliation":[{"name":"Civil Engineering Department Universidad de Concepci\u00f3n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Roa","sequence":"additional","affiliation":[{"name":"Civil Engineering Department Universidad de Concepci\u00f3n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Civil Engineering Department Universidad de Concepci\u00f3n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Nancucheo","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda Universidad San Sebasti\u00e1n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6474-2973","authenticated-orcid":false,"given":"Alex","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Civil Engineering Department Universidad de Concepci\u00f3n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsestengg.4c00563"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.70015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1201\/EBK1439804063"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511541490"},{"key":"e_1_2_8_6_1","first-page":"75","volume-title":"A Review of Sulphate Removal Options for Mine Waters","author":"Bowell R. 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