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With the ability to demonstrate complex 3D structure and multicellular interactions, organoids have advanced studies in all major organs as a reliable model. In this study, we generated an advanced cardiovascular organoid by using a genome\u2010edited human pluripotent stem cell line with inducible\n                    <jats:italic>SOX17<\/jats:italic>\n                    expression, enabling controlled endothelial specification, adjustable cell\u2010type composition, and human heart\u2010like morphology. Our organoids recapitulated the cardiotoxic phenotypes of FDA\u2010approved chemotherapeutic doxorubicin, manifesting as decreased cell viability and diminished contractile activity. Cryoinjury\u2010induced myocardial infarction in our organoids led to reduced beating, viability, and \u03b1\u2010actinin expression, along with increased fibroblast formation, which were mitigated by Captopril. Lastly, isoproterenol treatment increased peak Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transient amplitude and shortened APD\n                    <jats:sub>50<\/jats:sub>\n                    in our organoids, consistent with previously reported \u03b2\u2010adrenergic responses. In summary, we established a protocol for generating in vitro 3D cardiovascular organoids with controllable cellular composition and heart\u2010like structures, providing a robust and easy\u2010to\u2010produce platform for future studies of human heart disease.\n                  <\/jats:p>","DOI":"10.1002\/bit.70275","type":"journal-article","created":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T18:56:22Z","timestamp":1782068182000},"page":"2655-2667","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cardiovascular Organoids With Adjustable Endothelial Composition via SOX17\u2010Engineered hPSCs"],"prefix":"10.1002","volume":"123","author":[{"given":"Po\u2010Yu","family":"Liang","sequence":"first","affiliation":[{"name":"Davidson School of Chemical Engineering Purdue University West Lafayette Indiana USA"},{"name":"Purdue Institute for Cancer Research West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyuhyung","family":"Jin","sequence":"additional","affiliation":[{"name":"Davidson School of Chemical Engineering Purdue University West Lafayette Indiana USA"},{"name":"Purdue Institute for Cancer Research West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan R.","family":"Petrucci","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun Lance","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University University Park Pennsylvania USA"},{"name":"The Huck Institutes of the Life Sciences The Pennsylvania State University University Park Pennsylvania USA"},{"name":"Department of Biology The Pennsylvania State University University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Bao","sequence":"additional","affiliation":[{"name":"Davidson School of Chemical Engineering Purdue University West Lafayette Indiana USA"},{"name":"Purdue Institute for Cancer Research West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064561"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-006-9001-z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10062"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-016-0003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34730-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4087"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3LC00829K"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02571-7"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/20417314231190147"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3609"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-024-01526-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ams2.284"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14124"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","unstructured":"Ergir E. 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