{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:51:23Z","timestamp":1789487483812,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The biopharmaceutical industry increasingly relies on single\u2010use technologies (SUT) for operational flexibility. For a long time, SUT was considered more sustainable than stainless\u2010steel (SST) systems due to water and energy savings. This study re\u2010evaluates this paradigm via a detailed bottom\u2010up analysis of the SUT carbon footprint at the 2000\u2009L scale. Our analysis, based on two real\u2010world facility case studies (a full\u2010SUT and a hybrid\u2010SUT model), shows that the CO2 footprint of SUT is significantly higher than previously assumed. This granular analysis, based on physical disassembly and updated \u201ccradle\u2010to\u2010gate\u201d factors, identifies filters and bags as key emission hotspots. A separate comparative analysis also shows that key SST process steps\u2014favored by the progressive decarbonization of electricity grids\u2014can now have a lower carbon footprint than their SUT counterparts. Our data demonstrates that an operationally optimized hybrid facility design, which combines SUT with SST, can significantly reduce plastic waste and associated emissions. These findings compel a reassessment of sustainability strategies in biopharmaceutical manufacturing and highlight the potential of fit\u2010for\u2010purpose hybrid models as an effective lever for reducing the ecological footprint.<\/jats:p>","DOI":"10.1002\/bit.70297","type":"journal-article","created":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T16:59:48Z","timestamp":1783270788000},"page":"2802-2814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Revisiting the Carbon Footprint of Single\u2010Use Technologies in Biomanufacturing: A Bottom\u2010Up Analysis Reveals a Paradigm Shift"],"prefix":"10.1002","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3294-0916","authenticated-orcid":false,"given":"Jan","family":"Reiners","sequence":"first","affiliation":[{"name":"Roche Diagnostics GmbH Penzberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Bruno\u2010Thakur","sequence":"additional","affiliation":[{"name":"F. Hoffmann\u2010La Roche Ltd. Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daina","family":"Romeo","sequence":"additional","affiliation":[{"name":"F. Hoffmann\u2010La Roche Ltd. Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"Ku\u015bmierczyk","sequence":"additional","affiliation":[{"name":"Lonza Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinand","family":"St\u00fcckler","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH Penzberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.52825\/isec.v1i.1043"},{"key":"e_1_2_11_3_1","volume-title":"Non\u2010level Playing Field for Renewable Materials vs. Fossil in Life Cycle Assessments. 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