{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T20:39:28Z","timestamp":1789331968450,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2024LNYKJ23"],"award-info":[{"award-number":["2024LNYKJ23"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    ) is a leading cause of urinary tract infections (UTIs). The escalating misuse of antibiotics has led to the emergence of multidrug\u2010resistant strains, necessitating novel therapeutic approaches. Bacteriophages and their encoded lytic enzymes offer unique advantages for antimicrobial therapy and show promising potential for future applications. This study focuses on the characterization of uropathogenic\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    phages and their lytic enzymes, investigating their biological properties and potential therapeutic applications. We isolated a novel lytic bacteriophage, vB_PaeS_PA16c (PPA16c), which demonstrated favorable biological characteristics, including a 34% biofilm eradication capacity within 6\u2009h. The phage\u2010encoded endolysin PA16cLys exhibited superior antibacterial efficacy compared to the phage itself. Notably, it achieved significant biofilm reduction (~63%) after 1\u2009h treatment without requiring outer membrane permeabilizers. This endolysin exhibited enhanced antibacterial efficacy in eradicating mature\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    biofilms. Regarding host range, PA16cLys displayed broader lytic activity. Both the phage and endolysin demonstrated cross\u2010order lytic activity against other pathogenic bacteria. Crucially, PA16cLys maintained potent inhibitory activity against phage\u2010resistant mutants. These findings provide a theoretical foundation for utilizing phages and lytic enzymes against uropathogenic\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and establish a research basis for developing novel antimicrobial therapy.\n                  <\/jats:p>","DOI":"10.1002\/bit.70301","type":"journal-article","created":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T17:02:04Z","timestamp":1783270924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization and Therapeutic Potential of a Novel Lytic Phage\u2010Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms"],"prefix":"10.1002","author":[{"given":"Shilin","family":"Zhang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"},{"name":"R&amp;D Center, Aim Honesty Biopharmaceutical Co. 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Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Niu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"},{"name":"Jimo District Market Supervision and Administration Bureau Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaru","family":"Hong","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruibing","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid","family":"Shahbaz","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansong","family":"You","sequence":"additional","affiliation":[{"name":"R&amp;D Center, Aim Honesty Biopharmaceutical Co. Ltd. Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizhong","family":"Quan","sequence":"additional","affiliation":[{"name":"Liaoning U&amp;ME Future Technology Co. Ltd. Shenyang\u2010Fushun Reform and Innovation Demonstration District Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguang","family":"Li","sequence":"additional","affiliation":[{"name":"Liaoning U&amp;ME Future Technology Co. Ltd. Shenyang\u2010Fushun Reform and Innovation Demonstration District Liaoning China"},{"name":"Department of Animal Husbandry and Veterinary Medicine Liaoning Agricultural Vocational and Technical College Yingkou Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-8888","authenticated-orcid":false,"given":"Xiaoyu","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Xu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6621-404X","authenticated-orcid":false,"given":"Lili","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Bio\u2010Intelligent Manufacturing, School of Bioengineering Dalian University of Technology Dalian Liaoning China"},{"name":"Liaoning U&amp;ME Future Technology Co. 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Shenyang\u2010Fushun Reform and Innovation Demonstration District Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.70956"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(03)00061-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04931.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499248"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1093668"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16030478"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2023.199184"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00434-16"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb06050.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10060310"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina59091686"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00293"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2020.08.014"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0042-6822(03)00172-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2510.181699"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00362-12"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2020.02.017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.01.005"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297920050053"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03786-14"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2020.09.061"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10060338"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2209383119"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12101076"},{"key":"e_1_2_9_26_1","unstructured":"National Health Commission of the People's Republic of China. \u201cMinistry of Education of the People's Republic of China Ministry of Science and Technology of the People's Republic of China National Administration of Traditional Chinese Medicine.\u201d Measures for Ethical Review of Life Sciences and Medical Research Involving Human Subjects. Beijing China;2023 Retrieved from.https:\/\/www.nhc.gov.cn\/qjjys\/c100016\/202302\/6b6e447b3edc4338856c9a652a85f44b.shtml."},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-019-03071-4"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24931"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3013"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-011-9844-5"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00477-5"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.01.009"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.796132"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5c00142"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2019.296"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2147\/RRU.S273663"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.108"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.0c03558"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.70301","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T17:02:06Z","timestamp":1783270926000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.70301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,5]]},"references-count":38,"alternative-id":["10.1002\/bit.70301"],"URL":"https:\/\/doi.org\/10.1002\/bit.70301","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,5]]},"assertion":[{"value":"2026-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"bit.70301"}}