{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T10:16:37Z","timestamp":1783332997115,"version":"3.54.6"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00dcrkiye Bilimsel ve Teknolojik Ara\u015et\u0131rma Kurumu","doi-asserted-by":"publisher","award":["119C054"],"award-info":[{"award-number":["119C054"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004410","name":"T\u00dcrkiye Bilimsel ve Teknolojik Ara\u015et\u0131rma Kurumu","doi-asserted-by":"publisher","award":["217S118"],"award-info":[{"award-number":["217S118"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Overexpression of epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) is common in solid tumors like breast, colorectal, and head and neck cancers, driving oncogenic signaling, therapy resistance, and poor prognosis. Monotherapies often fall short in fully suppressing tumor progression, particularly in cases with EGFR\/HER2 co\u2010amplification. Dual\u2010targeting strategies offer enhanced efficacy by mitigating resistance and amplifying antitumor responses. Our study focused on developing and characterizing a bispecific anti\u2010EGFR\/HER2 antibody designed to simultaneously block ligand binding and receptor activation while harnessing Fc\u2010mediated immune effector functions. Utilizing knob\u2010into\u2010hole and CrossMab technologies, we engineered a bispecific antibody capable of binding the extracellular domains of both EGFR and HER2.\n                    <jats:italic>In vitro<\/jats:italic>\n                    analyses confirmed its dual\u2010binding capacity. Comparative studies revealed that this bispecific construct outperformed its monospecific counterparts in suppressing proliferation and, notably, in increasing the expression of apoptotic markers in EGFR\/HER2\u2010expressing tumor cells. Together, these findings highlight the synergistic therapeutic potential of bispecific antibodies as a promising modality for overcoming the limitations of current monotherapies also supporting the fight against drug resistance.\n                  <\/jats:p>","DOI":"10.1002\/bit.70304","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T09:43:47Z","timestamp":1783331027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Developing Anti\u2010EGFR\/Anti\u2010HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4678-6152","authenticated-orcid":false,"given":"Senem","family":"Sen","sequence":"first","affiliation":[{"name":"Materials Science and Nanotechnology Graduate Program Bilkent University Ankara Turkey"},{"name":"Nobel Pharmaceuticals Biotechnology R&amp;D, Marmara Teknokent Kocaeli 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