{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:02:51Z","timestamp":1785330171147,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To increase the information content in early stages of bioprocess development, various technologies have been introduced for monitoring cultivations in microtiter plates (MTPs). Besides spectroscopical measurements, like in the BioLector technology, optical measurements with luminescent sensors, placed directly in the cultivation medium, became widely established. Monitoring the oxygen transfer rate (OTR) is a key technology for bioprocess development, as it allows mass balancing. At the same time, it is a criterion for a rational scale\u2010up of cultivations. Although this technology has recently been transferred to monitoring of OTR in 96\u2010well MTPs, monitoring of other parameters like carbon dioxide transfer rate (CTR) in individual wells was so far impossible. In this study, the preparation and integration of novel CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010sensitive fluorescent sensors into the micro(\u00b5)\u2010scale Transfer rate Online Measurement device (\u00b5TOM) is reported, that rely on spectrally compatible diketopyrrolopyrrole indicator dye. By including 48 CO\n                    <jats:sub>2<\/jats:sub>\n                    and 48 O\n                    <jats:sub>2<\/jats:sub>\n                    sensors, the 96\u2010well sensor layout of the \u00b5TOM allows for simultaneous measurement of CTR and OTR in separate replicate wells and the calculation of 48 respiratory quotients (RQs). Additionally, to reduce the overall media consumption, liquid filling volumes between 0.2 and 0.5\u2009mL were applied. The first\u2010ever parallel monitoring of the OTR, CTR, and RQ in one MTP during cultivations of\n                    <jats:italic>Hansenula polymorpha<\/jats:italic>\n                    RB11 pC10\u2010FMD (P\n                    <jats:sub>FMD<\/jats:sub>\n                    \u2010GFP) and\n                    <jats:italic>E. coli<\/jats:italic>\n                    BL21(DE3) pRotHi\u2010YFP are presented. The interpretation of the RQ allowed distinguishing between the oxygen\u2010unlimited growth of\n                    <jats:italic>Hansenula polymorpha<\/jats:italic>\n                    on glucose, as well as the accumulation and consumption of ethanol. Furthermore, the RQ was used to identify the sequential consumption of glucose and glycerol by\n                    <jats:italic>E. coli<\/jats:italic>\n                    BL21(DE3) in Wilms\u2010MOPS autoinduction medium. Thus, the additional online parameters, CTR and RQ, extend the possibilities for early\u2010stage bioprocess development at MTP\u2010scale.\n                  <\/jats:p>","DOI":"10.1002\/bit.70322","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:19:24Z","timestamp":1785327564000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expanding Metabolic Online Monitoring Capabilities in 96\u2010Deepwell Microtiter Plates by Combining Optoelectronic O\n                    <sub>2<\/sub>\n                    and CO\n                    <sub>2<\/sub>\n                    Sensors"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6721-0868","authenticated-orcid":false,"given":"Robert","family":"Dinger","sequence":"first","affiliation":[{"name":"Chair of Biochemical Engineering (AVT.BioVT) RWTH Aachen University Aachen North Rhine Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Institute of Analytical Chemistry and Food Chemistry Graz University of Technology Graz Styria Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Flitsch","sequence":"additional","affiliation":[{"name":"PyroScience GmbH Aachen North Rhine Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan P.","family":"Fischer","sequence":"additional","affiliation":[{"name":"PyroScience GmbH Aachen North Rhine Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"Lattermann","sequence":"additional","affiliation":[{"name":"Kuhner Shaker GmbH Herzogenrath North Rhine Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9318-8273","authenticated-orcid":false,"given":"Sergey M.","family":"Borisov","sequence":"additional","affiliation":[{"name":"Institute of Analytical Chemistry and Food Chemistry Graz University of Technology Graz Styria Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2012-3476","authenticated-orcid":false,"given":"Jochen","family":"B\u00fcchs","sequence":"additional","affiliation":[{"name":"Chair of Biochemical Engineering (AVT.BioVT) RWTH Aachen University Aachen North Rhine Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-703X(00)00116-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-703X(03)00181-5"},{"key":"e_1_2_9_4_1","volume-title":"Biochemical Engineering and Biotechnology Handbook","author":"Atkinson 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