{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:12:43Z","timestamp":1791292363654,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tangential flow filtration\n                    <jats:bold>(<\/jats:bold>\n                    TFF) perfusion using hollow\u2010fiber modules offers substantial advantages over other mammalian cell retention technologies. Recently, increased awareness of Starling flow has helped to enhance filtration performance to similar, or even higher levels compared to alternative technologies. Despite these improvements, scaling TFF perfusion remains challenging and often requires time\u2010consuming trial\u2010and\u2010error efforts. This manuscript presents a systematic, data\u2010driven four\u2010step workflow for designing and scaling TFF perfusion systems, integrating hydraulic considerations, bubble removal studies, and pump characterizations. While filter selection in TFF perfusion often receives significant attention, the design of the cell retention loop is commonly overlooked. However, this element plays a crucial role in system performance and can cause suboptimal pump operation, residence time issues, and bubble accumulation. The developed data\u2010driven workflow facilitates the optimization of designs for generic TFF perfusion systems utilizing hollow\u2010fiber modules, effectively bridging the gap between small\u2010scale and manufacturing\u2010scale implementations.\n                  <\/jats:p>","DOI":"10.1002\/bit.70335","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T05:49:43Z","timestamp":1786513783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A General Workflow for Tangential Flow Filtration Perfusion Scale\u2010Up"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7555-0598","authenticated-orcid":false,"given":"Patrick","family":"Romann","sequence":"first","affiliation":[{"name":"Levitronix GmbH Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Lee","sequence":"additional","affiliation":[{"name":"AstraZeneca Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7552-337X","authenticated-orcid":false,"given":"Venkatesh","family":"Natarajan","sequence":"additional","affiliation":[{"name":"Amgen Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9214-8511","authenticated-orcid":false,"given":"Daniel","family":"Ley","sequence":"additional","affiliation":[{"name":"Novo Nordisk Hiller\u00f8d Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0939-701X","authenticated-orcid":false,"given":"Claes Nymand","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Novo Nordisk Hiller\u00f8d Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Garcia\u2010Munzer","sequence":"additional","affiliation":[{"name":"Novartis Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8115-4874","authenticated-orcid":false,"given":"Dominik","family":"Schieman","sequence":"additional","affiliation":[{"name":"Novartis Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8173-8749","authenticated-orcid":false,"given":"Mahbod","family":"Hajighasemi","sequence":"additional","affiliation":[{"name":"Merck &amp; Co., Inc. 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