{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T18:25:34Z","timestamp":1786904734098,"version":"build-2736575974"},"reference-count":100,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Food security and environmental sustainability have emerged as major global challenges, driving the search for alternative protein production systems that can meet growing demand while reducing the environmental burden of conventional livestock farming. Among emerging alternatives, cultivated meat has gained considerable attention due to its potential to produce animal\u2010derived protein without the need for large\u2010scale animal rearing and slaughter. Although cultivated meat remains absent from most commercial markets owing to technical, economic, and regulatory challenges, significant milestones have been achieved, including regulatory approval in Singapore in 2020, completion of the U.S. Food and Drug Administration pre\u2010market safety consultation in 2022, and authorization for commercial sale by the U.S. Department of Agriculture in 2023. Substantial progress has been made in cell sourcing, cell\u2010line engineering, serum\u2010free culture media development, scaffold fabrication, and bioprocess optimization. However, large\u2010scale production of cultivated meat with desirable texture, flavor, nutritional quality, and economic viability remains challenging. Key bottlenecks include the development of cost\u2010effective animal\u2010component\u2010free media, scalable bioreactor systems, edible scaffold materials, and efficient downstream processing strategies. This review provides an updated assessment of recent advances in cultivated meat technology, encompassing cell\u2010line development, scaffold engineering, bioprocessing, post\u2010processing approaches, sustainability considerations, and regulatory frameworks. Furthermore, current technological limitations, commercialization challenges, and future research priorities are discussed to evaluate the potential role of cultivated meat within sustainable and resilient future food systems.<\/jats:p>","DOI":"10.1002\/bit.70347","type":"journal-article","created":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T18:15:24Z","timestamp":1786904124000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advances in Cultivated Meat Technology for Sustainable Alternative Protein Production: A Review"],"prefix":"10.1002","author":[{"given":"Zaryab","family":"Shafi","sequence":"first","affiliation":[{"name":"Department of Biosciences Integral University Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2819-6298","authenticated-orcid":false,"given":"Pinku Chandra","family":"Nath","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Architecture Techno India University Agartala Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9818-5805","authenticated-orcid":false,"given":"Vinay Kumar","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Graphic Era (Deemed to be University) Dehradun Uttarakhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8610-1026","authenticated-orcid":false,"given":"Mohd. Zobair","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Environmental Microbiology Babasaheb Bhimrao Ambedkar University Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumira","family":"Malik","sequence":"additional","affiliation":[{"name":"School of Basic and Applied Sciences K. R. Mangalam University Gurgaon Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10408398.2025.2528723","article-title":"Cultivated Meat Quality: A Systematic Review on the Physicochemical, Technological and Sensory Evaluation","volume":"66","author":"Agostinis Ferreira G.","year":"2026","journal-title":"Critical Reviews in Food Science and Nutrition"},{"issue":"2","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"622","DOI":"10.3390\/nu13020622","article-title":"Omega\u20103 Polyunsaturated Fatty Acids Prevent Nonalcoholic Steatohepatitis (NASH) and Stimulate Adipogenesis","volume":"13","author":"Antraco V. 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