{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T20:26:36Z","timestamp":1787084796920,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000340835"],"award-info":[{"award-number":["RS\u20102024\u201000340835"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Resolvin E2 (RvE2; 5\n                    <jats:italic>S<\/jats:italic>\n                    ,18\n                    <jats:italic>R<\/jats:italic>\n                    \u2010dihydroxyeicosapentaenoic acid) and 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010resolvin D5 (17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010RvD5; 7\n                    <jats:italic>S<\/jats:italic>\n                    ,17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010dihydroxydocosahexaenoic acid) are specialized pro\u2010resolving mediators (SPMs) that actively promote the resolution of inflammation. However, their efficient biocatalytic production remains challenging because of the limited catalytic performance and poor stability of previously reported 5\n                    <jats:italic>S<\/jats:italic>\n                    \u2010lipoxygenases (5\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOXs). Here, we identified and characterized a novel double\u2010oxygenating 15\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOX from\n                    <jats:italic>Pseudobdellovibrionaceae bacterium<\/jats:italic>\n                    exhibiting 5\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOX activity and evaluated its potential as a whole\u2010cell biocatalyst for SPM production. Recombinant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    cells expressing this enzyme stereoselectively converted 18\n                    <jats:italic>R<\/jats:italic>\n                    \u2010hydroxyeicosapentaenoic acid (18\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HEPE) and 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010hydroxydocosahexaenoic acid (17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HDHA), prepared using engineered 18\n                    <jats:italic>R<\/jats:italic>\n                    \u2010 and 15\n                    <jats:italic>R<\/jats:italic>\n                    \u2010LOXs from\n                    <jats:italic>Sorangium cellulosum<\/jats:italic>\n                    , into RvE2 and 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010RvD5, respectively. Product identities were confirmed by chiral\u2010phase HPLC and LC\u2013MS\/MS analyses. Reaction conditions, including pH, temperature, and DMSO, cell, and substrate concentrations, were optimized to enhance biotransformation performance. Under optimized conditions, 4.0\u2009mM 18\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HEPE and 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010HDHA were converted into 0.57\u2009mM (190.61\u2009mg\/L) RvE2 and 1.48\u2009mM (533.5\u2009mg\/L) 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010RvD5 within 60 and 30\u2009min, respectively. RvE2 production was 2.4\u2010fold higher than that obtained using a previously reported purified 5\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOX, and this study provides the first quantitative report of 17\n                    <jats:italic>R<\/jats:italic>\n                    \u2010RvD5 production. These findings establish a double\u2010oxygenating 15\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOX with 5\n                    <jats:italic>S<\/jats:italic>\n                    \u2010LOX activity as an efficient whole\u2010cell platform for SPM production.\n                  <\/jats:p>","DOI":"10.1002\/bit.70355","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T19:42:41Z","timestamp":1787082161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole\u2010Cell Production of Resolvin E2 and 17\n                    <i>R<\/i>\n                    \u2010Resolvin D5 by a Double\u2010Oxygenating 15\n                    <i>S<\/i>\n                    \u2010Lipoxygenase With 5\n                    <i>S<\/i>\n                    \u2010Lipoxygenase Activity From\n                    <i>Pseudobdellovibrionaceae bacterium<\/i>"],"prefix":"10.1002","author":[{"given":"Hee\u2010Gyeong","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Bioscience and Biotechnology Konkuk University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung\u2010Chul","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology Hankuk University of Foreign Studies Yongin\u2010Si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6886-7589","authenticated-orcid":false,"given":"Deok\u2010Kun","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology Konkuk University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8473com"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00416.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12363"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2014.07.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0370-1"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2026.107064"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27820"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28995"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4gc00308j"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.137713"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.6b02870"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103652"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048775y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28997"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2005.03.175"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020760"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr100396c"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-022-04150-w"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.0c02922"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-015-2694-4"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609212200"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1011944"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.09.011"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1GC00561H"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800550n"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70355","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.70355","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.70355","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T19:42:42Z","timestamp":1787082162000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.70355"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":26,"alternative-id":["10.1002\/bit.70355"],"URL":"https:\/\/doi.org\/10.1002\/bit.70355","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]},"assertion":[{"value":"2026-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"bit.70355"}}