{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:28:32Z","timestamp":1788535712461,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["18\/epsrc\u2010cdt\/3587"],"award-info":[{"award-number":["18\/epsrc\u2010cdt\/3587"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["101052376"],"award-info":[{"award-number":["101052376"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell\u2010surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells. Five key glycogenes (COSMC, FUT8, \u03b24GALT1, ST3GAL4, and ST6GAL1) were investigated in two mAb\u2010producing CHO cell lines. Product glycan analysis revealed consistent, gene\u2010specific effects across hosts, including loss of core fucosylation, and tunable galactosylation and sialylation. Lectin\u2010based surface profiling reliably reflected product outcomes for COSMC and FUT8 modifications but showed limited correspondence with product galactosylation and \u03b12,3\u2010sialylation phenotypes, highlighting glycosylation pathway redundancy and context dependence. These findings demonstrate that lectin\u2010based profiling is most effective for identifying glycoengineering outcomes associated with nonredundant pathways rather than as a universal predictor of product glycosylation.<\/jats:p>","DOI":"10.1002\/bit.70359","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T08:08:56Z","timestamp":1788163736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unmasking Glycoforms: Lectin\u2010Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells"],"prefix":"10.1002","author":[{"given":"Cristina","family":"Abascal Ruiz","sequence":"first","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6317-3797","authenticated-orcid":false,"given":"Sheryl Li Yan","family":"Lim","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4943-7233","authenticated-orcid":false,"given":"Jacobus","family":"Brink","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Carillo","sequence":"additional","affiliation":[{"name":"National Institute for Bioprocessing Research &amp; Training, Characterisation and Comparability Laboratory Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6471-7356","authenticated-orcid":false,"given":"Eoin","family":"Casey","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8978-2592","authenticated-orcid":false,"given":"Jonathan","family":"Bones","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"},{"name":"National Institute for Bioprocessing Research &amp; Training, Characterisation and Comparability Laboratory Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9289-0191","authenticated-orcid":false,"given":"Ioscani","family":"Jim\u00e9nez del Val","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Bioprocess Engineering University College Dublin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201800111"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201700748"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20094"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.1c00689"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2018.07.015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-019-0288-4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.05.023"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023266"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20200840"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2477"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338352"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwr027"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(01)01301-3"},{"key":"e_1_2_9_15_1","unstructured":"ICH. 1999.ICH Q6B Specifications: Test Procedures and Acceptance Criteria for Biotechnological\/Biological Products. 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