{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:16:06Z","timestamp":1788156966268,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Generation of CAR\u2010T cell therapies relies predominantly on lentiviral vectors (LVs). As in vivo CAR\u2010T trials progress, demand for larger quantities of higher\u2010potency LV is expected. However, primary capture by anion\u2010exchange chromatography (AIEX) remains a major manufacturing bottleneck from use of adsorbents not tailored to LVs, leading to complex binding phenomena such as time\u2010dependent product loss and a heterogenous two\u2010peak elution profile arising from two distinct LV subpopulations (Peak 1 and Peak 2). Current industry practice is to collect both peaks to maximize total product recovery. Yet peak\u2010specific differences in product potency and demand for higher purity products for use in in vivo CAR\u2010T cell therapy will likely drive a need to separate these LV species. Electrospun nanofibers offer a promising next\u2010generation adsorbent for addressing these challenges, making it essential to understand how Q\u2010nanofiber design affects LV recovery and Peak 1\/Peak 2 selectivity. This work indicates that Peak 1 and Peak 2 LVs differ in their T\u2010cell transduction potency. Despite generating higher numbers of CD3\n                    <jats:sup>+<\/jats:sup>\n                    GFP\n                    <jats:sup>+<\/jats:sup>\n                    T cells at low doses, Peak 1 sees reducing T\u2010cell viability and %CD3\n                    <jats:sup>+<\/jats:sup>\n                    GFP\n                    <jats:sup>+<\/jats:sup>\n                    cells at higher doses, likely from an inhibitory effect of VSV\u2010G vesicles that are co\u2010eluted with Peak 1 LV during AIEX. In contrast, Peak 2 LV maintained increasing %CD3\n                    <jats:sup>+<\/jats:sup>\n                    GFP\n                    <jats:sup>+<\/jats:sup>\n                    T cells and high viability across doses. A Q\u2010nanofiber adsorbent enabling high\u2010recovery AIEX while increasing LV peak separation was therefore required to isolate the more potent Peak 2 LV subpopulation. Increasing nanofiber porosity (0.4\u20130.6\u2009v\/v) enhanced LV recovery by reducing time\u2010dependent loss, whereas Peak Separation increased with ligand density and nanofiber diameter. An optimal Q\u2010nanofiber structure was therefore identified and validated across two LV batches, achieving high recovery (64%\u201382%) while isolating Peak 2 LV from the major VSV\u2010G elution.\n                  <\/jats:p>","DOI":"10.1002\/bit.70360","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:42:40Z","timestamp":1788154960000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolating T\u2010Cell Potent Lentiviral Vector Subpopulations through Rational Design of Nanofiber Anion\u2010Exchange Adsorbents"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9016-1716","authenticated-orcid":false,"given":"George","family":"Pamenter","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering University College London London UK"},{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Ovari","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering University College London London UK"},{"name":"Cytiva Stevenage UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurag","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2854-5351","authenticated-orcid":false,"given":"Thomas","family":"Williams\u2010Fegredo","sequence":"additional","affiliation":[{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Knevelman","sequence":"additional","affiliation":[{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriacos","family":"Mitrophanous","sequence":"additional","affiliation":[{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9361-2651","authenticated-orcid":false,"given":"Lee","family":"Davies","sequence":"additional","affiliation":[{"name":"Oxford Biomedica (UK) Ltd Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzad","family":"Foroutan","sequence":"additional","affiliation":[{"name":"Cytiva Stevenage UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3942-3790","authenticated-orcid":false,"given":"Matthew","family":"Townsend","sequence":"additional","affiliation":[{"name":"Cytiva Stevenage UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8319-1679","authenticated-orcid":false,"given":"Stephen","family":"Goldrick","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering University College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3866-3304","authenticated-orcid":false,"given":"Daniel G.","family":"Bracewell","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering University College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2025.132365"},{"key":"e_1_2_9_3_1","unstructured":"Alliance for Regenerative Medicine. \u201c2024 State of the Industry Briefing\u201d2024."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2009.10.017"},{"key":"e_1_2_9_5_1","unstructured":"Astrea Bioseparations2025. \u201cLentiHERO\u00ae.\u201d Accessed July 1 2025.https:\/\/www.astreabioseparations.com\/en\/products\/AstreAdept%C2%AE-Chromatography-Capsules\/lentihero-lentivirus-purification\/lentihero\/?srsltid=AfmBOopj6YHCEN1kzWnj2s87zGQ9cG0twZt0iWz10scqIwRc0iEP9wT1."},{"issue":"2","key":"e_1_2_9_6_1","first-page":"2019","article-title":"Residual Bootstraps for Regression Model Validation","volume":"14","author":"Ayoade A. 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