{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T07:16:29Z","timestamp":1788333389403,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Autotrophic CO\n                    <jats:sub>2<\/jats:sub>\n                    conversion to Single Cell Protein (SCP) using H\n                    <jats:sub>2<\/jats:sub>\n                    and O\n                    <jats:sub>2<\/jats:sub>\n                    is gaining commercial traction for food and feed production.\n                    <jats:italic>Cupriavidus necator<\/jats:italic>\n                    H16 is a key chassis organism for this \u201cknallgas\u201d fermentation, but industrial operation is typically constrained to ~30\u00b0C due to poor autotrophic growth at higher temperatures. Autotrophic growth at 37\u00b0C would enable the use of low\u2010cost cooling water instead of chilled water, offering substantial reduction in cooling expenses. Here, we characterised a spontaneous\n                    <jats:italic>C. necator<\/jats:italic>\n                    mutant that conferred 37\u00b0C\u2010thermotolerance when grown autotrophically, and compared its performance to the wild type. In batch cultures at 30\u00b0C, both strains showed comparable growth. At 37\u00b0C, only the spontaneous mutant grew autotrophically, albeit with a lower maximum growth rate than at 30\u00b0C. In chemostat cultivations no clear phycological differences could be resolved within the experimental uncertainty relative to the wild type at 30\u00b0C, in terms of PHB production, CO\n                    <jats:sub>2<\/jats:sub>\n                    uptake at 37\u00b0C, and proteinogenic amino acid composition. Techno\u2010economic modelling of a 160\u2009kt a\n                    <jats:sup>\u22121<\/jats:sup>\n                    SCP process indicated that operation at 37\u00b0C can reduce cooling utility costs up to 75%, saving ~US$ 0.98 per kg SCP (20%\u201350% of the production costs). Operating at 37\u00b0C with thermotolerant strains could subsequently decrease production costs substantially, making CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010derived SCP production more economically viable at scale.\n                  <\/jats:p>","DOI":"10.1002\/bit.70366","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T06:58:25Z","timestamp":1788332305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reducing Cooling Costs for Sustainable Food Biomanufacturing With Thermotolerant\n                    <i>Cupriavidus necator<\/i>\n                    H16 Mutant"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4450-3630","authenticated-orcid":false,"given":"Lars","family":"Puiman","sequence":"first","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology The University of Queensland St. Lucia Queensland Australia"},{"name":"ARC Centre of Excellence in Synthetic Biology The University of Queensland St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6752-9043","authenticated-orcid":false,"given":"James Kemp","family":"Heffernan","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology The University of Queensland St. Lucia Queensland Australia"},{"name":"ARC Centre of Excellence in Synthetic Biology The University of Queensland St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Marcellin","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology The University of Queensland St. Lucia Queensland Australia"},{"name":"ARC Centre of Excellence in Synthetic Biology The University of Queensland St. Lucia Queensland Australia"},{"name":"Queensland Metabolomics and Proteomics Q\u2010MAP The University of Queensland St. Lucia Queensland Australia"},{"name":"Integrated Design Environment for Advanced biomanufacturing (IDEA Bio) The University of Queensland St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Cupriavidus Necator as a Model Organism for CO2\u2010based Biotechnology","author":"Alhafiz H. 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