{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T06:57:34Z","timestamp":1790578654357,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LTGS24B060002"],"award-info":[{"award-number":["LTGS24B060002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The plasticity of the enzyme active site is widespread in naturally evolved homologous enzymes, but has been less explored in engineered enzymes. In this study, the plasticity of the active site in sucrose phosphorylases (SPs) for 3\u2010O\u2010\u03b1\u2010glucopyranosyl\u2010resveratrol synthesis was obtained. The mutant R135L\/Y344Q of\n                    <jats:italic>Bl<\/jats:italic>\n                    SP from\n                    <jats:italic>Bifidobacterium lactis<\/jats:italic>\n                    showed the same catalytic activity with the previous mutant Q345F, but displayed different microenvironment of active site and enzymatic properties. The new mutant could almost completely convert up to 15\u2009g\/L resveratrol into resveratrol glycoside without cosolvent, meanwhile, generate a new impurity 3\u2010O\u2010\u03b1\u2010diglucoside\u2010resveratrol, which was identified as 3\u2010O\u2010\u03b1\u2010maltosyl\u2010resveratrol.\n                  <\/jats:p>","DOI":"10.1002\/bit.70383","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T06:41:16Z","timestamp":1790577676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Remodeling the Active Site of Sucrose Phosphorylase for Effective Resveratrol Glycosylation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7170-502X","authenticated-orcid":false,"given":"Changxin","family":"Lu","sequence":"first","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"},{"name":"College of Chemical Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaibin","family":"Du","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"He","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0505-1010","authenticated-orcid":false,"given":"Hanchi","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7077-160X","authenticated-orcid":false,"given":"Linjiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3869-352X","authenticated-orcid":false,"given":"Xiaolong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering, State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou China"},{"name":"Institute of Fermentation Engineering Zhejiang University of Technology Hangzhou 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