{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:01:42Z","timestamp":1789995702526,"version":"4.0.1"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Enzyme screening platforms are typically evaluated based on their throughput and analytical performance rather than their ability to mimic the conditions encountered at large scale. In bioreactors, enzymes experience gradients in substrate concentration, oxygen availability, pH, temperature, and interfacial exposure, caused by imperfect mixing and multiphase operation. These gradients affect both enzyme activity and stability, yet they are rarely incorporated into the assays used to select biocatalysts. Traditionally, a variant is chosen based on its performance under static, well\u2010mixed laboratory conditions, yet those conditions do not necessarily represent the process conditions it must eventually perform under. Microfluidic devices can in principle recreate process\u2010relevant conditions at a small scale since these devices offer precise control over the flow, residence time, and multiphase contact. Yet most current platforms are not built to mimic the scale\u2010down process\u2010derived conditions. Here, we assess current microfluidic tools against this process\u2010relevant conditions and outline how modular designs can be combined into sequential screening platforms that generate the conditions in a bioreactor, for early\u2010stage biocatalyst selection.<\/jats:p>","DOI":"10.1002\/bit.70387","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:05:57Z","timestamp":1789992357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Scale\u2010Down Platforms for Process\u2010Oriented Enzyme Screening Using Microfluidics"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9637-1070","authenticated-orcid":false,"given":"Maria I.","family":"Rodriguez\u2010Torres","sequence":"first","affiliation":[{"name":"Department of Chemical and Biochemical Engineering Technical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1411-9793","authenticated-orcid":false,"given":"Elif","family":"Erdem","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biochemical Engineering Technical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3514-5731","authenticated-orcid":false,"given":"Junhyeong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7774-7442","authenticated-orcid":false,"given":"Ulrich","family":"Kr\u00fchne","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biochemical Engineering Technical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2016-4091","authenticated-orcid":false,"given":"Michael","family":"Daniele","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering North Carolina State University Raleigh North Carolina USA"},{"name":"Lampe Joint Department of Biomedical Engineering North Carolina State University and University of North Carolina Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7976-2483","authenticated-orcid":false,"given":"John M.","family":"Woodley","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biochemical Engineering Technical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2022.100733"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2025.07.010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RE00483A"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.108071"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2020.04.010"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cite.202200177"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp050249q"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4NP00031E"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.5c04752"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-025-00428-5"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac102472a"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50497b"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2019.03.016"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5RE00489F"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.checat.2022.09.037"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.4c05265"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","unstructured":"Gantz M. S. V.Mathis F. E. H.Nintzel et al.2024. Microdroplet Screening Rapidly Profiles a Biocatalyst to Enable its AI\u2010assisted Engineering. bioRxiv 2024.04.08.588565: Cold Spring Harbor Laboratory.https:\/\/doi.org\/10.1101\/2024.04.08.588565.","DOI":"10.1101\/2024.04.08.588565"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00910"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.202100159"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2018.09.001"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-019-02294-z"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.202500038"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2024.120282"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00717H"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi13081363"},{"key":"e_1_2_6_27_1","first-page":"400","article-title":"EnzymeML: Seamless Data Flow and Modeling of Enzymatic Data","volume":"20","author":"Lauterbach S.","year":"2023","journal-title":"Nature Methods"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338677"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-024-01261-x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf8761"},{"key":"e_1_2_6_31_1","unstructured":"Marques S. 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