{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:58:38Z","timestamp":1790351918665,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000104","name":"National Aeronautics and Space Administration","doi-asserted-by":"publisher","award":["80NSSC23K0584"],"award-info":[{"award-number":["80NSSC23K0584"]}],"id":[{"id":"10.13039\/100000104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2209217"],"award-info":[{"award-number":["2209217"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Long\u2010duration human missions to Mars will require autonomous systems capable of converting in situ resources into structural materials, tools, and functional components. More broadly, such systems represent a class of resource\u2010limited bioprocesses relevant to extreme\u2010environment manufacturing. Here, we investigate synthetic autotrophic\u2013heterotrophic consortia, inspired by lichen biology, as a platform for autonomous biofabrication from granular feedstocks. We experimentally screened filamentous fungi and paired them with diazotrophic cyanobacteria to identify mutually supportive consortia capable of sustained growth and biomineral production in the presence of Martian regolith simulant as the primary inorganic substrate, without external organic carbon or nitrogen inputs. Selected co\u2010cultures exhibited evidence of metabolic coupling, and untargeted metabolomic analysis revealed coordinated reprogramming consistent with integrated carbon and nitrogen metabolism within the consortia. These systems facilitated mineral consolidation of regolith particles, demonstrating the feasibility of near\u2013closed\u2010loop biomineral production under resource\u2010limited conditions. While integration with additive manufacturing remains conceptual, this study establishes a framework for engineering self\u2010sustaining microbial consortia for biomaterials production and highlights opportunities for coupling metabolism with material synthesis in both extraterrestrial and terrestrial environments.<\/jats:p>","DOI":"10.1002\/bit.70392","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:59:02Z","timestamp":1790348342000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Weaving Life Into Regolith: Synthetic Autotrophic\u2013Heterotrophic Consortia for Autonomous Biofabrication From Granular Feedstocks"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2277-4345","authenticated-orcid":false,"given":"Nisha","family":"Rokaya","sequence":"first","affiliation":[{"name":"Department of Plant Pathology University of Nebraska\u2013Lincoln Lincoln 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