{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:59:32Z","timestamp":1790351972688,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Synechococcus elongatus<\/jats:italic>\n                    PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO\n                    <jats:sub>2<\/jats:sub>\n                    . We found that a laboratory strain of\n                    <jats:italic>S. elongatus<\/jats:italic>\n                    PCC 7942 expressing the\n                    <jats:italic>ydeH<\/jats:italic>\n                    gene from\n                    <jats:italic>E. coli<\/jats:italic>\n                    exhibited pronounced biofilm formation and cell aggregation upon\n                    <jats:italic>ydeH<\/jats:italic>\n                    induction, despite the species rarely showing these phenotypes under standard laboratory conditions. Our findings demonstrate that this strain harbors latent potential for biofilm formation and aggregation through c\u2010di\u2010GMP synthesis, suggesting possible applications in biomass recovery. Further, we identified that\n                    <jats:italic>xcsA2<\/jats:italic>\n                    (Synpcc7942_2151), a putative cellulose synthase gene containing a PilZ domain that binds c\u2010di\u2010GMP is an indispensable gene in this biofilm\u2010producing phenotype and experimentally confirmed\n                    <jats:italic>ydeH<\/jats:italic>\n                    \u2010induced cellulose\u2010like \u03b2\u22121,4\u2010linked glucans production. Notably,\n                    <jats:italic>xcsA2<\/jats:italic>\n                    represents the first putative functional cellulose synthase gene involved in biofilm\/aggregate formation in\n                    <jats:italic>S. elongatus<\/jats:italic>\n                    and suggests that this organism may serve as a useful platform for bacterial cellulose bioproduction. Overall, discovery of\n                    <jats:italic>xcsA2<\/jats:italic>\n                    provides a new genetic basis for exploring cellulose synthase evolution in cyanobacteria and specifically opens possibilities for engineering cellulose bioproduction utilizing\n                    <jats:italic>S. elongatus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/bit.70396","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:01:31Z","timestamp":1790348491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Putative Cellulose Synthase Gene\n                    <i>xcsA2<\/i>\n                    Required for c\u2010di\u2010GMP\u2010Induced Biofilm Formation and Cell Aggregation in\n                    <i>Synechococcus elongatus<\/i>\n                    PCC 7942"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3299-2722","authenticated-orcid":false,"given":"Chihiro C.","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Life and Environmental System Science, Graduate School of Nanobioscience Yokohama City University Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3311-1511","authenticated-orcid":false,"given":"Jiro F.","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental System Science, Graduate School of Nanobioscience Yokohama City University Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2671-4645","authenticated-orcid":false,"given":"Shinsuke","family":"Kutsuna","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental System Science, Graduate School of Nanobioscience Yokohama City University Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8867-3546","authenticated-orcid":false,"given":"Robert A.","family":"Kanaly","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental System Science, Graduate School of Nanobioscience Yokohama City University Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00862-22"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00451-13"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01121"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.25712"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants14172655"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53113-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","unstructured":"Boitreaud J. 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