{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:47:55Z","timestamp":1791798475272,"version":"4.3.4"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp;amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Anaerobic fermentation for volatile fatty acids (VFAs) production represents the sustainable and resource\u2010recovery strategy for waste activated sludge (WAS) treatment, yet its efficiency is often hampered by low substrate C\/N ratio and limited hydrolysis.\n                    <jats:italic>Ulva<\/jats:italic>\n                    , a macroalga responsible for extensive \u201cgreen tides\u201d that burden coastal ecosystems and economies, emerges as an ideal co\u2010substrate for WAS anaerobic fermentation due to its high C\/N ratio and biodegradability. This study established a novel\n                    <jats:italic>Ulva<\/jats:italic>\n                    \u2013WAS co\u2010fermentation system to boost VFAs production and explained the mechanism of acidogenic enhancement and methanogenesis suppression through integrated analyses of VFAs generation, dissolved organic matter (DOM) molecular transformation, and microbial metabolic activity. Co\u2010fermentation enhanced VFAs production (1.67\u20132.9\u2010fold increase of maximum VFAs accumulation) while stimulating microbial activity and accelerating hydrolysis of macromolecular components into polysaccharides and amino acids. Within the carbohydrate\u2010supplemented environment, DOM transformation was dominated by microbial acidogenic processes, including glycolysis and amino acid metabolism. Microbial community succession and metabolic profiling confirmed that the high\u2010C\/N, carbohydrate\u2010rich fermentation environment drove deterministic assembly of communities toward the function of acidogenesis, which in turn further promoted VFAs production. Concurrently, reactive oxygen species (ROS) stress induced by VFAs accumulation selectively inhibited methanogenic activity. This work provides an innovative strategy for WAS treatment and offers profound insights into the interplay between DOM dynamics and microbial metabolism in co\u2010fermentation systems.\n                  <\/jats:p>","DOI":"10.1002\/bit.70410","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:11:53Z","timestamp":1791796313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Co\u2010Fermentation of\n                    <i>Ulva<\/i>\n                    and Waste Activated Sludge for Sustainable Resource Recovery: Mechanistic Insights From Dissolved Organic Matter Transformation and Multi\u2010Omics Analysis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6376-318X","authenticated-orcid":false,"given":"Lifeng","family":"Wang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Multi\u2010Media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering Huazhong University of Science and Technology Wuhan China"},{"name":"Green Energy Industry Research Centre (GEIRC) Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6546-9331","authenticated-orcid":false,"given":"Yibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Multi\u2010Media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering Huazhong University of Science and Technology Wuhan China"},{"name":"Green Energy Industry Research Centre (GEIRC) Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyan","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yazhi","family":"Nong","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Multi\u2010Media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering Huazhong University of Science and Technology Wuhan China"},{"name":"Green Energy Industry Research Centre (GEIRC) Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2595-5669","authenticated-orcid":false,"given":"Chenyuan","family":"Dang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Multi\u2010Media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering Huazhong University of Science and Technology Wuhan China"},{"name":"Green Energy Industry Research Centre (GEIRC) Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5054-571X","authenticated-orcid":false,"given":"Jie","family":"Fu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Multi\u2010Media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering Huazhong University of Science and Technology Wuhan China"},{"name":"Green Energy Industry Research Centre (GEIRC) Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2012.703"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.143007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apenergy.2014.02.035"},{"key":"e_1_2_9_5_1","volume-title":"Standard Methods for the Examination of Water and Wastewater","author":"American Public Health Association","year":"1926"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuel.2020.117494"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2021.106423"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wasman.2020.08.051"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0715-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2021.09.006"},{"key":"e_1_2_9_11_1","article-title":"Standard Methods for Examination of Water and Wastewater","author":"Carranzo I. 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