{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:44:27Z","timestamp":1791870267582,"version":"4.3.4"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Modified Vaccinia virus Ankara (MVA) is a potent vector for the development of vaccines and novel cancer treatment strategies. Since it is a non\u2010replicating virus in human recipients, high infective titers are required for therapeutic efficacy. Harvesting and clarification of cell culture\u2010derived MVA are critical for the overall process yield and remains challenging, as full MVA release depends on cell lysis, resulting in a difficult\u2010to\u2010filter cell lysate. In this study, we present the development of a high\u2010yield clarification strategy using diatomaceous earth (DE) as a natural filter aid, followed by an optimized endonuclease treatment.<\/jats:p>\n                  <jats:p>An ionic strength containing a 650\u2009mM salt mixture of NaCl, NaBr, and KCl allowed impurity depletion by DE binding while maintaining a complete virus recovery. The optimal alluvial filtration conditions were achieved with a PURAFIX\u00ae CH 09 depth filter with a 10\u201330\u2009\u00b5m retention rate. The optimal conditions were identified as a combination of a Celpure\u00ae C1000 precoat and a Celpure\u00ae C300 additive added to the feed. This robust process achieved 94% total virus recovery while reducing turbidity to 23% and residual DNA and protein impurities to 76% and 72%, respectively, in a single\u2010step filtration. Subsequent nuclease digestion benefits from a reduced particle and impurity burden, resulting in further 93% DNA depletion under optimal conditions of 11\u2009h of incubation at 21\u00b0C with 51\u2009U\/mL of the salt active DENARASE\u00ae High Salt endonuclease. The study demonstrated that sterilized filter aids enable a single\u2010step filtration to recover high MVA titers with a reduced impurity burden, paving the way for improved downstream process efficiency.<\/jats:p>","DOI":"10.1002\/bit.70413","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T04:46:30Z","timestamp":1791866790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1082-1702","authenticated-orcid":false,"given":"Linus G.","family":"Weber","sequence":"first","affiliation":[{"name":"Centre of Competence for Biotechnology and Biomedical Physics (BioTecMed), Institute of Bioprocess Engineering and Pharmaceutical Technology University of Applied Sciences Mittelhessen (THM) Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Stephan","sequence":"additional","affiliation":[{"name":"Centre of Competence for Biotechnology and Biomedical Physics (BioTecMed), Institute of Bioprocess Engineering and Pharmaceutical Technology University of Applied Sciences Mittelhessen (THM) Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9097-1991","authenticated-orcid":false,"given":"Georgios","family":"Makris","sequence":"additional","affiliation":[{"name":"FILTROX AG St. Gallen Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leander","family":"John","sequence":"additional","affiliation":[{"name":"ProBioGen AG Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2711-7252","authenticated-orcid":false,"given":"Ingo","family":"Jordan","sequence":"additional","affiliation":[{"name":"ProBioGen AG Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7443-3122","authenticated-orcid":false,"given":"Michael W.","family":"Wolff","sequence":"additional","affiliation":[{"name":"Centre of Competence for Biotechnology and Biomedical Physics (BioTecMed), Institute of Bioprocess Engineering and Pharmaceutical Technology University of Applied Sciences Mittelhessen (THM) Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"FDA. 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