{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T07:40:45Z","timestamp":1707896445575},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2003,1,29]],"date-time":"2003-01-29T00:00:00Z","timestamp":1043798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The aim of this study was to investigate the importance of somatostatin receptor subtype 2 (SSTR2) expression for 111In-labelled diethylenetriamine-pentaacetic acid (DTPA)-d-Phe1-octreotide binding and uptake of 111In in neuroendocrine tumours.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>111In activity concentrations in surgical biopsies from neuroendocrine tumours (midgut carcinoid and medullary thyroid carcinoma), breast carcinoma and blood were determined 1\u20138 days after intravenous injection of 111In-labelled DTPA-d-Phe1-octreotide (140\u2013350 MBq). The ratio of 111In activity concentrations between tumour tissue and blood (T\/B value) was calculated. The expression of SSTR2 messenger RNA (mRNA) in tumour biopsies was quantitated by ribonuclease protection assay and SSTR2 protein was localized by immunocytochemistry.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>T\/B values were highest for tumour biopsies from midgut carcinoids (mean 160 (range 4\u20131200); n = 65) followed by medullary thyroid carcinoma (mean 38 (range 2\u2013350); n = 88) and breast carcinoma (mean 18 (range 4\u201341); n = 4). The expression of SSTR2 mRNA (relative to the NCI-H69 cell line) was highest in tumour biopsies from midgut carcinoids (mean 2\u00b75 (range 0\u00b783\u20136\u00b70); n = 40) followed by medullary thyroid carcinoma (mean 1\u00b73 (range 0\u00b720\u20136\u00b70); n = 7) and breast carcinoma (mean 0\u00b766 (range 0\u00b729\u20131\u00b70); n = 9). In tumour biopsies SSTR2 protein was localized exclusively to tumour cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Midgut carcinoid tumours showed a much higher level of SSTR2 expression than medullary thyroid carcinoma in accordance with superior tumour imaging by octreotide scintigraphy. The high SSTR2 mRNA values and T\/B values observed in midgut carcinoid tumours were positively correlated.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/bjs.4069","type":"journal-article","created":{"date-parts":[[2003,5,2]],"date-time":"2003-05-02T10:01:45Z","timestamp":1051869705000},"page":"549-554","source":"Crossref","is-referenced-by-count":5,"title":["111In-labelled octreotide binding by the somatostatin receptor subtype 2 in neuroendocrine tumours"],"prefix":"10.1093","volume":"90","author":[{"given":"S H","family":"Hashemi","sequence":"first","affiliation":[{"name":"Lundberg Laboratory for Cancer Research at the Department of Pathology, G\u00f6teborg University, G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S-A","family":"Benjeg\u00e5rd","sequence":"additional","affiliation":[{"name":"Lundberg Laboratory for Cancer Research at the Department of Radiation Physics, G\u00f6teborg University, G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Ahlman","sequence":"additional","affiliation":[{"name":"Lundberg Laboratory for Cancer Research at the Department of Surgery, G\u00f6teborg University, G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"W\u00e4ngberg","sequence":"additional","affiliation":[{"name":"Lundberg Laboratory for Cancer Research at the Department of Surgery, G\u00f6teborg University, G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Forssell-Aronsson","sequence":"additional","affiliation":[{"name":"Lundberg Laboratory for Cancer Research at the Department of Radiation Physics, G\u00f6teborg University, G\u00f6teborg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Billig","sequence":"additional","affiliation":[{"name":"Department of Physiology, G\u00f6teborg University, G\u00f6teborg, 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