{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T07:55:42Z","timestamp":1769846142603,"version":"3.49.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2008,1,14]],"date-time":"2008-01-14T00:00:00Z","timestamp":1200268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"Chinese National Basic Research and Development Grants \u2018973\u2019","doi-asserted-by":"publisher","award":["2004CB518801"],"award-info":[{"award-number":["2004CB518801"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"Chinese National Basic Research and Development Grants \u2018973\u2019","doi-asserted-by":"publisher","award":["2002CB713804"],"award-info":[{"award-number":["2002CB713804"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012164","name":"Chinese High-Tech Programme \u2018863\u2019","doi-asserted-by":"publisher","award":["2003AA216080"],"award-info":[{"award-number":["2003AA216080"]}],"id":[{"id":"10.13039\/501100012164","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Recovery of the surgically damaged mesothelial cell layer is a major process in reducing postoperative peritoneal adhesions. Sphingosine kinase (SPK) 1 is a signalling molecule involved in the regulation of proliferation and migration of various cell types. This study determined the effect of SPK-1 gene transfer on the recovery of damaged mesothelial cells and on peritoneal adhesion formation after surgery.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Rat mesothelial cells were isolated and characterized by their expression of cytokeratin and vimentin. Their migration was determined by scratch wound motility assay. Cellular SPK-1 activity was measured by [\u03b3-32P]adenosine 5\u2032-triphosphate incorporation. Wistar rats underwent laparotomy with subsequent caecum or uterine horn abrasion. Rats were randomized to either SPK-1 gene (Ad-SPK-1) transfer or control groups. The animals were killed 14 days after operation and peritoneal adhesions were graded.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Adenovirus-mediated SPK-1 gene transfer increased the cellular SPK-1 activity of mesothelial cells, leading to enhanced migration. Median adhesion scores were significantly lower in the Ad-SPK-1 group than in controls in both rat caecum (0\u00b798 versus 2\u00b760; P &amp;lt; 0\u00b7001) and rat uterine horn (0\u00b728 versus 1\u00b783; P &amp;lt; 0\u00b7001) models.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Adenovirus-mediated SPK-1 gene transfer promotes recovery of the surgically damaged mesothelial cell layer and prevents postoperative peritoneal adhesion formation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/bjs.5890","type":"journal-article","created":{"date-parts":[[2007,12,6]],"date-time":"2007-12-06T19:46:39Z","timestamp":1196970399000},"page":"252-258","source":"Crossref","is-referenced-by-count":14,"title":["Sphingosine kinase 1 gene transfer reduces postoperative peritoneal adhesion in an experimental model"],"prefix":"10.1093","volume":"95","author":[{"given":"Q","family":"Guo","sequence":"first","affiliation":[{"name":"Department of General Surgery, General Hospital of People's Liberation Army, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q-F","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Experimental Haematology, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H-J","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Experimental Haematology, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery, General Hospital of People's Liberation Army, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C-T","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Experimental Haematology, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L-S","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Experimental Haematology, Beijing Institute of Radiation Medicine , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,2,1]]},"reference":[{"key":"2024093006383886400_bib1","first-page":"60","article-title":"Intestinal obstruction from adhesions\u2014how big is the problem?","volume":"72","author":"Menzies","year":"1990","journal-title":"Ann R Coll Surg Engl"},{"key":"2024093006383886400_bib2","first-page":"147","article-title":"Postoperative adhesions: their treatment and relevance in 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