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This experimental study investigated the role of Rho-kinase in hepatectomy-induced sensitization to endotoxin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL\/6J mice underwent 68 per cent hepatectomy and were injected 24 h later with 100 \u00b5g Escherichia coli lipopolysaccharide (LPS). Simultaneously, animals received either fasudil or Y-27632 for Rho-kinase inhibition, or phosphate-buffered saline. Untreated hepatectomized animals served as positive controls and sham-operated animals as negative controls. Liver injury and inflammatory parameters were assessed 6 h after LPS challenge by serum alanine aminotransferase (ALT) levels, histomorphology and enzyme-linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hepatectomy resulted in a significant susceptibility to LPS, as indicated by inflammatory leucocyte recruitment (mean(s.e.m.) 10(1) leucocytes per high-power field), hepatocellular disintegration (ALT 22\u00b74(3\u00b71) \u00b5kat\/l) and apoptotic cell death (3\u00b78(0\u00b72) per cent). Rho-kinase inhibition reduced leucocytic infiltration by more than 33 per cent, abolished hepatocellular apoptosis entirely, and reduced tumour necrosis factor \u03b1 expression by more than 48 per cent and CXC chemokine expression by more than 36 per cent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hepatectomy increased susceptibility to LPS by Rho-kinase-dependent mechanisms. Blocking Rho-kinase signalling decreased LPS-induced liver injury in hepatectomized mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bjs.6082","type":"journal-article","created":{"date-parts":[[2008,6,18]],"date-time":"2008-06-18T15:33:17Z","timestamp":1213803197000},"page":"976-984","source":"Crossref","is-referenced-by-count":11,"title":["Rho-kinase signalling mediates endotoxin hypersensitivity after partial hepatectomy"],"prefix":"10.1093","volume":"95","author":[{"given":"J E","family":"Slotta","sequence":"first","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University, Malm\u00f6, Sweden"},{"name":"Institute for Clinical and Experimental Surgery, University of Saarland, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M W","family":"Laschke","sequence":"additional","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University, Malm\u00f6, Sweden"},{"name":"Institute for Clinical and Experimental Surgery, University of Saarland, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M D","family":"Menger","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Surgery, University of Saarland, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Thorlacius","sequence":"additional","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University, Malm\u00f6, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,6,18]]},"reference":[{"key":"2021070907425568700_bib1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/00000658-199909000-00004","article-title":"Clinical score for predicting recurrence after hepatic resection for metastatic colorectal cancer: analysis of 1001 consecutive cases","volume":"230","author":"Fong","year":"1999","journal-title":"Ann Surg"},{"key":"2021070907425568700_bib2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1136\/gut.2004.046524","article-title":"The value of residual liver volume as a predictor of hepatic dysfunction and infection after major liver resection","volume":"54","author":"Schindl","year":"2005","journal-title":"Gut"},{"key":"2021070907425568700_bib3","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1111\/j.1478-3231.2006.01342.x","article-title":"Role of Kupffer cells in host defense and liver disease","volume":"26","author":"Bilzer","year":"2006","journal-title":"Liver Int"},{"key":"2021070907425568700_bib4","first-page":"668","article-title":"Changes in the reticuloendothelial phagocytic function after partial hepatectomy","volume":"105","author":"Arii","year":"1985","journal-title":"J Lab Clin Med"},{"key":"2021070907425568700_bib5","first-page":"1428","article-title":"Liver failure induces a systemic inflammatory response. 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