{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:14:35Z","timestamp":1791980075227,"version":"4.4.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T00:00:00Z","timestamp":1325808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The adhesive mechanisms regulating leucocyte\u2013endothelium interactions in the pancreas remain elusive, but selectins may play a role. This study examined the molecular mechanisms mediating leucocyte rolling along the endothelium in the pancreas and the therapeutic potential of targeting the rolling adhesive interaction in acute pancreatitis (AP).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Pancreatitis was induced by retrograde infusion of 5 per cent sodium taurocholate into the pancreatic duct, repeated intraperitoneal administration of caerulein (50 \u00b5g\/kg) or intraperitoneal administration of l-arginine (4 g\/kg) in C57BL\/6 mice. A control and a monoclonal antibody against P-selectin were administered before and after induction of AP. Serum and tissue were sampled to assess the severity of pancreatitis, and intravital microscopy was used to study leucocyte rolling.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Taurocholate infusion into the pancreatic duct increased the serum level of trypsinogen, trypsinogen activation, pancreatic neutrophil infiltration, macrophage inflammatory protein (MIP) 2 formation and tissue damage. Immunoneutralization of P-selectin decreased the taurocholate-induced increase in serum trypsinogen (median (range) 17\u00b735 (12\u00b720\u201330\u00b700) versus 1\u00b755 (0\u00b760\u201315\u00b770) \u00b5g\/l; P = 0\u00b7017), neutrophil accumulation (4\u00b700 (0\u00b775\u20134\u00b700) versus 0\u00b763 (0\u20133\u00b725); P = 0\u00b7002) and tissue damage, but had no effect on MIP-2 production (14\u00b708 (1\u00b768\u201333\u00b738) versus 3\u00b770 (0\u00b755\u201351\u00b780) pg\/mg; P = 0\u00b7195) or serum trypsinogen activating peptide level (1\u00b710 (0\u00b760\u20131\u00b760) versus 0\u00b745 (0\u20131\u00b780) \u00b5g\/l; P = 0\u00b7069). Intravital fluorescence microscopy revealed that anti-P-selectin antibody inhibited leucocyte rolling completely in postcapillary venules of the inflamed pancreas.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Inhibition of P-selectin protected against pancreatic tissue injury in experimental pancreatitis. Targeting P-selectin may be an effective strategy to ameliorate inflammation in AP.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/bjs.7775","type":"journal-article","created":{"date-parts":[[2011,11,23]],"date-time":"2011-11-23T09:46:13Z","timestamp":1322041573000},"page":"246-255","source":"Crossref","is-referenced-by-count":60,"title":["P-selectin mediates neutrophil rolling and recruitment in acute pancreatitis"],"prefix":"10.1093","volume":"99","author":[{"given":"H","family":"Hartman","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Abdulla","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Awla","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Lindkvist","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Jeppsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Thorlacius","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Regn\u00e9r","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Malm\u00f6, Section of Surgery, Sk\u00e5ne University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,2,1]]},"reference":[{"key":"2024092904520151000_bib1","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.3748\/wjg.v12.i13.1985","article-title":"Co-localization hypothesis: a mechanism for the intrapancreatic activation of digestive enzymes during the early phases of acute pancreatitis","volume":"12","author":"van Acker","year":"2006","journal-title":"World J 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