{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T21:22:42Z","timestamp":1769203362954,"version":"3.49.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":244,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"JSPS KAKENHI","award":["16 K11349"],"award-info":[{"award-number":["16 K11349"]}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["18ek0109258h0002"],"award-info":[{"award-number":["18ek0109258h0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["19ek0109258h0003"],"award-info":[{"award-number":["19ek0109258h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Biliary atresia is a rare paediatric biliary obliteration disease with unknown aetiology, and is the most common indication for paediatric liver transplantation (LT). However, no consensus for predicting Kasai portoenterostomy (KP) outcomes using liver histological findings exists. Ki67 is a popular biomarker for measuring and monitoring cellular proliferation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Ki67 (clone, MIB-1) liver parenchyma expression was measured by immunohistochemical staining of samples from living donors and patients with biliary atresia to assess its value in predicting outcomes after KP.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 35 children with biliary atresia, 13 were native liver survivors (NLS), 17 were non-NLS, and five had primary LT. The median proportion of Ki67 immunostained areas in donors and patients with biliary atresia at KP was 0\u00b706 and 0\u00b799 per cent respectively. Univariable analysis identified a high proportion of Ki67 areas, high Ki67 cell numbers and high Ki67-positive\/leucocyte common antigen-positive cell numbers at KP as significant predictors of poor native liver survival after KP (hazard ratio 9\u00b729, 3\u00b737 and 12\u00b717 respectively). The proportion of Ki67 areas in the non-NLS group was significantly higher than that in the NLS group (1\u00b729 versus 0\u00b772 per cent respectively; P\u00a0=\u00a00\u00b7001), and then decreased at LT (0\u00b732 per cent versus 1\u00b729 per cent at KP; P &amp;lt; 0\u00b7001).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This study has demonstrated the clinical data and time course of Ki67 expression in patients with biliary atresia. High Ki67 expression at KP may be an important predictor of native liver survival following the procedure.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/bjs5.50308","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T14:35:28Z","timestamp":1592318128000},"page":"873-883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ki67 expression at Kasai portoenterostomy as a prognostic factor in patients with biliary atresia"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-7711","authenticated-orcid":false,"given":"D","family":"Yoshii","sequence":"first","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Inomata","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"},{"name":"Department of Paediatric Surgery and Transplantation , Kumamoto Rosai Hospital, Yatsushiro, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Komohara","sequence":"additional","affiliation":[{"name":"Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Shimata","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Hayashida","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Oya","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hibi","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery and Transplantation, Kumamoto University Graduate School of Medical Sciences , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,6,16]]},"reference":[{"key":"2023020208004269300_bjs550308-bib-0001","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1016\/S0140-6736(09)60946-6","article-title":"Biliary atresia","volume":"374","author":"Hartley","year":"2009","journal-title":"Lancet"},{"key":"2023020208004269300_bjs550308-bib-0002","first-page":"5","article-title":"Treatment of biliary atresia with special reference to hepatic porto-enterostomy and its modifications","volume":"6","author":"Kasai","year":"1974","journal-title":"Prog Pediatr Surg"},{"key":"2023020208004269300_bjs550308-bib-0003","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1136\/adc.56.6.460","article-title":"Hepatic portoenterostomy for biliary atresia. 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