{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T14:34:33Z","timestamp":1700577273698},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T00:00:00Z","timestamp":1323648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem Molecular Bio Educ"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the current laboratory assignment, technical aspects of the polymerase chain reaction (PCR) are integrated in the context of six different bacterial outbreak scenarios. The \u201cEnterobacterial Repetitive Intergenic Consensus Sequence\u201d (ERIC) PCR was used to analyze different outbreak scenarios. First, groups of 2\u20134 students determined optimal ERIC\u2010PCR conditions to validate the protocol and subsequently applied ERIC\u2010PCR to identify genetic relatedness among bacterial strains. Based on these genetic fingerprints, students selected the outbreak cases from the patient samples and assessed the risk factors for the outbreak scenario. Finally, students presented their findings during a classroom presentation. The results indicated that the assignment successfully facilitated student learning on the technical aspects of (ERIC) PCR and clearly demonstrated the practical application of PCR in a clinical diagnostic setting. Additionally, the assignment was highly appreciated by the students.<\/jats:p>","DOI":"10.1002\/bmb.20562","type":"journal-article","created":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T12:22:06Z","timestamp":1323692526000},"page":"112-120","source":"Crossref","is-referenced-by-count":0,"title":["Molecular diagnostic analysis of outbreak scenarios"],"prefix":"10.1002","volume":"40","author":[{"given":"M C.","family":"Morsink","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. E.","family":"Dekter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Dirks\u2010Mulder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. B.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,12]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"17","volume-title":"Molecular Diagnostics: Techniques & Applications","author":"Van Leeuwen WB","year":"2009"},{"key":"e_1_2_6_3_2","unstructured":"Folder Minor Molecular Diagnostics:http:\/\/www.hsleiden.nl\/aems\/home\/Molecular102011.pdf"},{"key":"e_1_2_6_4_2","first-page":"43","volume-title":"Molecular Microbiology: Diagnostic Principles and Practice","author":"Persing DH","year":"2004"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02505024"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","DOI":"10.4324\/9781410618054","volume-title":"Ten Steps to Complex Learning: A Systematic Approach to Four\u2010Component Instructional Design","author":"Van Merri\u00ebnboer JJG","year":"2007"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_2_6_8_2","first-page":"43","article-title":"Streamlining assessment","volume":"38","author":"Bres M","year":"2009","journal-title":"J. Coll. Sci. Teach."},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/advan.90179.2008"},{"key":"e_1_2_6_10_2","first-page":"337","article-title":"Centers for Disease Control and Prevention (2009) Cholera outbreak\u2014Southern Sudan","volume":"58","year":"2007","journal-title":"MMWR"},{"key":"e_1_2_6_11_2","first-page":"929","article-title":"Centers for Disease Control and Prevention (2008) Outbreak of Salmonella serotype Saintpaul infections associated with multiple raw produce items\u2014United States","volume":"57","journal-title":"MMWR"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa042859"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/502384"},{"key":"e_1_2_6_14_2","first-page":"445","volume-title":"Microbiology, An Introduction","author":"Tortora","year":"2007"},{"key":"e_1_2_6_15_2","first-page":"194","article-title":"Centers for Disease Control and Prevention (2009) Methicillin\u2010resistant Staphylococcus aureus skin infections from an elephant calf\u2014San Diego, California","volume":"58","year":"2008","journal-title":"MMWR"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-86702009000500005"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.1997.00162.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03440.x"},{"key":"e_1_2_6_19_2","first-page":"121","volume-title":"Molecular Diagnostics; Fundamentals, Methods & Clinical Applications","author":"Buckingham","year":"2007"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-6241-4_6"}],"container-title":["Biochemistry and Molecular Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmb.20562","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.20562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T13:48:36Z","timestamp":1700574516000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bmb.20562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,12]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/bmb.20562"],"URL":"https:\/\/doi.org\/10.1002\/bmb.20562","archive":["Portico"],"relation":{},"ISSN":["1470-8175","1539-3429"],"issn-type":[{"value":"1470-8175","type":"print"},{"value":"1539-3429","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,12]]}}}