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However, recent studies have demonstrated an expanding and medically relevant role for <jats:italic>TAS2R38<\/jats:italic>. The AVI variant of T2R38 is associated with an increased risk of both colorectal cancer and <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\u2010associated sinus infection and T2R38 variants have been implicated in off\u2010target drug responses. To address ethical concerns associated with continued student <jats:italic>TAS2R38<\/jats:italic> gene testing, we developed an alternative to the traditional laboratory genotyping exercise. Instead of determining their own genotype, introductory level students isolated plasmid DNA containing a section of the human <jats:italic>TAS2R38<\/jats:italic> gene from <jats:italic>Escherichia coli<\/jats:italic>. Following PCR\u2010mediated amplification of a section of the <jats:italic>TAS2R38<\/jats:italic> gene spanning the SNP at position 785, students determined their assigned genotype by restriction enzyme digestion and agarose gel electrophoresis. Using the course wide genotype and phenotype data, students found that there was an association between <jats:italic>TAS2R38<\/jats:italic> genotype and the age of persistent <jats:italic>P. aeruginosa<\/jats:italic> acquisition in cystic fibrosis \u201cpatients.\u201d Assessment data demonstrated that students taking part in this new <jats:italic>TAS2R38<\/jats:italic> laboratory activity made clear learning gains. \u00a9 2015 by The International Union of Biochemistry and Molecular Biology, 43(2):100\u2013109, 2015.<\/jats:p>","DOI":"10.1002\/bmb.20846","type":"journal-article","created":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T06:23:06Z","timestamp":1424413386000},"page":"100-109","source":"Crossref","is-referenced-by-count":3,"title":["An alternative laboratory designed to address ethical concerns associated with traditional <i>TAS2R38<\/i> student genotyping"],"prefix":"10.1002","volume":"43","author":[{"given":"Michelle L.","family":"LaBonte","sequence":"first","affiliation":[{"name":"Department of the History of Science Harvard University Cambridge Massachusetts 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa A.","family":"Beers","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Wellesley College Wellesley Massachusetts 02481"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80705-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80706-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr293"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.6.080604.162340"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020464"},{"key":"e_1_2_7_7_1","first-page":"982","article-title":"Variations in bitter\u2010taste receptor genes, dietary intake, and colorectal adenoma risk. 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