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In the present study, the iodine number of fat extracted using the Folch method from cow milk, goat milk, chicken egg yolk and coconut milk was determined using a simple colorimetric procedure that comprises an iodine solution in ethanol and a starch solution, replacing the Wijs reagent and conventional titration procedures with sodium thiosulfate standard solutions. Resembling the Folch method, a low\u2010cost alternative approach, utilizing kerosene oil, isopropyl alcohol, and water, which can be used to extract fat from commonly consumed liquid food items, was also developed in the present study as a simple and reliable approach. The extracted fat from the proposed method can be sent to a biochemistry laboratory to determine the iodine number using the proposed 96\u2010well plate\u2010based colorimetric procedure. The proposed methods are suitable for laboratories in low\u2010income settings.<\/jats:p>","DOI":"10.1002\/bmb.21891","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T11:36:05Z","timestamp":1739446565000},"page":"305-310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Combining the <scp>F<\/scp>olch method with a simplified alternative fat extraction technique for use in a colorimetric assay to measure the degree of unsaturation in fat samples in a biochemistry laboratory"],"prefix":"10.1002","volume":"53","author":[{"given":"Vimoshini","family":"Pragash","sequence":"first","affiliation":[{"name":"Department of Biomedical Science BMS School of Science  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9393-9516","authenticated-orcid":false,"given":"Meran Keshawa","family":"Ediriweera","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200600012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(02)00253-6"},{"key":"e_1_2_10_4_1","volume-title":"Lehninger principles of biochemistry","author":"Cox MM","year":"2008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-016-0401-1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lipd.12079"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-998-0003-1"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-998-0227-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.201700396"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664-021-00168-x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuel.2012.10.011"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2017.03.029"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.01.036"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0843-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/6360487"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937404.2012.710134"},{"key":"e_1_2_10_18_1","first-page":"135","article-title":"Fatty acid profile of milk\u2010a review","volume":"57","author":"Markiewicz\u2010K\u0119szycka M","year":"2013","journal-title":"J Vet Res"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13041111"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S002202991000004X"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr8040402"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0396.2007.00714.x"}],"container-title":["Biochemistry and Molecular Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.21891","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T16:55:02Z","timestamp":1747673702000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bmb.21891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,13]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/bmb.21891"],"URL":"https:\/\/doi.org\/10.1002\/bmb.21891","archive":["Portico"],"relation":{},"ISSN":["1470-8175","1539-3429"],"issn-type":[{"value":"1470-8175","type":"print"},{"value":"1539-3429","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,13]]},"assertion":[{"value":"2024-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}