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Other limiting factors include access to equipment and reagents, resulting in students frequently working in pairs or small groups to complete experiments, and consequently, students gain limited experience in the practical techniques. In addition, due to competing subjects and a filled curriculum, there is typically limited or no opportunity for students to repeat a laboratory exercise. As a result, the time pressure forces students to focus on completing the laboratory exercise without engaging at a deeper level with the concepts and principles being demonstrated. This can result in deficiencies in student understanding principles as well as developing the required proficiency in hands\u2010on skills with equipment. To overcome these issues, we implemented an online laboratory data simulator to replicate the laboratory quantification of ethanol in simulated driver blood samples. This approach allowed students to attempt the exercise as often and whenever they chose, while still faithfully replicating a traditional laboratory setting. We implemented this approach across two Australian universities using a mixed\u2010methods approach and assessed the impact of the simulator on student learning of biochemistry lab\u2010related concepts. Based on our findings, we suggest that this online approach can effectively teach fundamental laboratory concepts to students while eliminating many of the constraints of hands\u2010on laboratory classes.<\/jats:p>","DOI":"10.1002\/bmb.21903","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T08:54:48Z","timestamp":1748940888000},"page":"370-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Teaching second\u2010year biochemistry students the principles of an enzyme\u2010catalyzed spectrophotometric assay with an online lab simulator"],"prefix":"10.1002","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3640-7414","authenticated-orcid":false,"given":"Matthew","family":"Clemson","sequence":"first","affiliation":[{"name":"School of Life and Environmental Sciences The University of Sydney  Sydney New South Wales 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