{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:45:05Z","timestamp":1790196305362,"version":"4.1.0"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biochem Molecular Bio Educ"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We describe a classroom\u2010ready bio\u2010layer interferometry protocol for upper\u2010level undergraduates that contrasts a macromolecular interaction with a small\u2010molecule interaction on an Octet R8. Biotinylated bovine serum albumin (BSA) is immobilized on Super Streptavidin sensors to enable two assays in parallel: (i) binding of a commercially available anti\u2010BSA IgG (protein\u2013protein) and (ii) binding of a long\u2010chain fatty acid such as palmitate (protein\u2013small molecule). The exercise emphasizes assay design decisions that differ by analyte class\u2014sensor chemistry and loading density, solvent matching and reference subtraction, association\/dissociation timing, and the choice of data\u2010analysis pathway (global 1:1 kinetic fitting for IgG vs. steady\u2010state isotherms for fatty acids). Representative data yield nanomolar K\n                    <jats:sub>D<\/jats:sub>\n                    values for BSA\u2013IgG and micromolar apparent K\n                    <jats:sub>D<\/jats:sub>\n                    for BSA\u2013fatty acid, with correspondingly larger response amplitudes and slower off\u2010rates for the protein\u2013protein case. The protocol fits into two 3\u2010h sessions, leverages eight\u2010channel parallel acquisition for efficient titrations, and includes safety notes, optional regeneration to demonstrate sensor reuse, and troubleshooting for non\u2010ideal behaviors (drift, avidity, non\u2010specific binding). Assessment artifacts (rubrics, short\u2010answer prompts, and figure\u2010quality criteria) support measurement of learning outcomes in experimental design and quantitative analysis. Because the same BSA surface underpins both assays, the module is cost effective, reproducible, and readily transferable across institutions, providing a practical framework for teaching how analyte size and mechanism shape biosensor strategy and interpretation.\n                  <\/jats:p>","DOI":"10.1002\/bmb.70049","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T04:23:39Z","timestamp":1774326219000},"page":"297-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Teaching Bio\u2010Layer Interferometry: Protein\u2013Protein Versus Protein\u2013Small Molecule Interaction Analysis on an Octet\n                    <scp>R8<\/scp>"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7387-4532","authenticated-orcid":false,"given":"Wen","family":"Chen","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering South China University of Technology (SCUT)  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"AG SBiolayer Interferometry for Label\u2010Free Biomolecular Interaction Analysis https:\/\/www.sartorius.com\/en\/products\/biolayer\u2010interferometry#:~:text=Biolayer%20Interferometry%20 throughput%20analysis%20capabilities."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138620709789104915"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmb.20442"},{"key":"e_1_2_11_5_1","unstructured":"P.Azadi \u201cBioLayer Interferometry (BLI) \u201dhttps:\/\/ast.uga.edu\/biolayer\u2010interferometry\u2010bli\/#:~:text=The%20BLI%20approach%20shares%20some Figure%201."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2024.117741"},{"key":"e_1_2_11_7_1","unstructured":"A. G.SartoriusandL.Dubey \u201cSartorius Launches the Octet R8e System: Real\u2010Time Label\u2010Free Biomolecular Interaction Analysis With Unprecedented Sensitivity and Efficiency \u201d2025 https:\/\/www.sartorius.com\/en\/company\/newsroom\/product\u2010news\/sartorius\u2010launches\u2010the\u2010octet\u2010r8e\u2010system\u2010real\u2010time\u2010label\u2010free\u2010biomolecular\u2010interaction\u2010analysis\u2010with\u2010unprecedented\u2010sensitivity\u2010and\u2010efficiency\u20101690702#:~:text=Leveraging%20Sartorius\u2019%20proprietary%20BLI%20technology scale%2C%20complexity%2C%20and%20cost%20requirements."},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2019.1623202"},{"key":"e_1_2_11_9_1","unstructured":"D.Apiyo(https:\/\/www.sartorius.com 2022)."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.5c03942"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)40546-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010024\u201001079\u20108"}],"container-title":["Biochemistry and Molecular Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.70049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmb.70049","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.70049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T09:52:44Z","timestamp":1779443564000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bmb.70049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,23]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/bmb.70049"],"URL":"https:\/\/doi.org\/10.1002\/bmb.70049","archive":["Portico"],"relation":{},"ISSN":["1470-8175","1539-3429"],"issn-type":[{"value":"1470-8175","type":"print"},{"value":"1539-3429","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,23]]},"assertion":[{"value":"2025-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}