{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:58:46Z","timestamp":1785463126704,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CHE\u20101555050"],"award-info":[{"award-number":["CHE\u20101555050"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CHE\u20102108278"],"award-info":[{"award-number":["CHE\u20102108278"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001309","name":"Research Corporation for Science Advancement","doi-asserted-by":"publisher","award":["22844"],"award-info":[{"award-number":["22844"]}],"id":[{"id":"10.13039\/100001309","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biochem Molecular Bio Educ"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Undergraduate laboratory courses should teach not only core experimental skills, but also the relevance of those skills to addressing contemporary scientific challenges. In this course, entitled Biochemistry &amp; Molecular Biology Laboratory, students clone a gene into an expression plasmid, express and purify an enzyme, and perform kinetic assays on the enzyme. The enzyme chosen for this experimental sequence is TEM\u20101, a \u03b2\u2010lactamase enzyme that is a key mediator of resistance to \u03b2\u2010lactam antibiotics, such as penicillins. In parallel, the students gain experience in microbiology and natural product extractions by first isolating a\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    species from soil and then screening this isolate for antibiotic activity and the ability to produce compounds that inhibit TEM\u20101 enzymatic activity. In principle, this research effort could lead to the discovery of either a novel strain of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptomyces clavuligerus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , which produces the important TEM\u20101 inhibitor clavulanate, or another\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    strain producing a novel TEM\u20101 inhibitor. Thus, students are introduced to a pressing problem in contemporary medicine, antibiotic resistance, while they participate in discovery\u2010driven efforts to solve that problem. Incorporation of discovery\u2010based objectives into the laboratory sequence gives students individualized datasets to analyze, thereby promoting the development of student analytical skills. In addition, during the course, the students enhance their scientific writing skills through a series of peer\u2010reviewed writing assignments and ultimately complete a formal journal\u2010style article, in which they communicate their research findings. Overall, this course compares favorably with a course\u2010based undergraduate research experience (CURE) course, as gauged by student\u2010reported learning gains and overall course assessment.\n                  <\/jats:p>","DOI":"10.1002\/bmb.70060","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:45:00Z","timestamp":1780634700000},"page":"368-376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Semester\u2010Long Laboratory in Molecular Biology and Biochemistry Focusing on the Discovery of Novel \u03b2\u2010Lactamase Inhibitors From\n                    <i>Streptomyces<\/i>"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4572-1050","authenticated-orcid":false,"given":"Matthew T.","family":"Gordon","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6698-4245","authenticated-orcid":false,"given":"R. M. Madhushi N.","family":"Ratnayake","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6350-9632","authenticated-orcid":false,"given":"Alix M.","family":"Rexford","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0925-4459","authenticated-orcid":false,"given":"Rebecca A.","family":"Butcher","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933\u2010951.2001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jmbe.v21i3.2157"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35021219"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00037\u201009"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2017.11.002"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106976.2000.tb00541.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.11.5.852"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.968053"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ed054p315"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ed300656p"},{"key":"e_1_2_11_12_1","first-page":"92","article-title":"The Science of Writing: Experimenting With Peer Review at a STEM University","volume":"48","author":"Mitchell T. J.","year":"2019","journal-title":"CEA Forum"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc10e04s19"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc10e01s18"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.6.1868\u20101871.2001"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm400769b"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06099\u201011"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi992505b"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1150788"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165351"},{"key":"e_1_2_11_22_1","unstructured":"D.Lopatto \u201cUndergraduate Research Experience Surveys \u201dn.d. sure.sites.grinnell.edu accessed November 11 2025."}],"container-title":["Biochemistry and Molecular Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.70060","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmb.70060","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmb.70060","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:16:51Z","timestamp":1785460611000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bmb.70060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/bmb.70060"],"URL":"https:\/\/doi.org\/10.1002\/bmb.70060","archive":["Portico"],"relation":{},"ISSN":["1470-8175","1539-3429"],"issn-type":[{"value":"1470-8175","type":"print"},{"value":"1539-3429","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]},"assertion":[{"value":"2024-10-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}