{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:22:19Z","timestamp":1773282139297,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,5,27]],"date-time":"2008-05-27T00:00:00Z","timestamp":1211846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rhizoma Anemarrhenae (<jats:italic>Zhimu<\/jats:italic> in Chinese), the dried rhizome of <jats:italic>Anemarrhena asphodeloides<\/jats:italic> Bge. (Fam. Liliaceae), is a well\u2010known traditional Chinese medicinal herb and has been used clinically in China for centuries to cure various diseases. However, like other traditional Chinese medicines, the effective constituents of this medicine, especially the assimilation and metabolites <jats:italic>in vivo<\/jats:italic>, which are very important to show their effects, have not been systematically studied. In this paper, solid\u2010phase extraction and liquid chromatography\u2013atmospheric pressure chemical ionization mass spectrometry technologies were used to study the constituents absorbed into rat urine and their metabolites after oral administration of Rhizoma Anemarrhenae decoction. A total of 11 compounds, including two xanthones, three of their metabolites and six steroidal saponins, were identified in rat urine sample. They were neomangiferin (<jats:bold>1<\/jats:bold>), glucuronide and monomethyl conjugate of mangiferin (<jats:bold>2<\/jats:bold>), mangiferin (<jats:bold>3<\/jats:bold>), monomethyl conjugate of mangiferin (<jats:bold>4<\/jats:bold>), dimethyl conjugate of mangiferin (<jats:bold>5<\/jats:bold>), timosaponin N or timosaponin E1 (<jats:bold>6<\/jats:bold>), timosaponin BII (<jats:bold>7<\/jats:bold>), timosaponin BIII (<jats:bold>8<\/jats:bold>), anemarrhenasaponin I or anemarrhenasaponin II (<jats:bold>9<\/jats:bold>), timosaponin AII (<jats:bold>10<\/jats:bold>) and timosaponin AIII (<jats:bold>11<\/jats:bold>). The results would efficaciously narrow the potentially active compounds range in Rhizoma Anemarrhenae decoction, and pave a helpful way for follow\u2010up mechanism of action research. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1026","type":"journal-article","created":{"date-parts":[[2008,5,27]],"date-time":"2008-05-27T12:58:19Z","timestamp":1211893099000},"page":"1066-1083","source":"Crossref","is-referenced-by-count":39,"title":["Identification of major xanthones and steroidal saponins in rat urine by liquid chromatography\u2013atmospheric pressure chemical ionization mass spectrometry technology following oral administration of Rhizoma Anemarrhenae decoction"],"prefix":"10.1002","volume":"22","author":[{"given":"Chunhui","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longxing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingsong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenggang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,27]]},"reference":[{"issue":"1","key":"e_1_2_1_2_1","first-page":"26","article-title":"Studies on the active constituents of Anemarrhena asphodeloides Bunge","volume":"27","author":"Dong JX","year":"1992","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.07.036"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.03.036"},{"issue":"6","key":"e_1_2_1_5_1","first-page":"473","article-title":"Isolation and structure determination of xanthone glycosides of Anemarrhena asphodeloides.","volume":"32","author":"Hong YF","year":"1997","journal-title":"Acta Pharmaceutica Sinica"},{"issue":"7","key":"e_1_2_1_6_1","first-page":"518","article-title":"Isolation and identification of steroidal saponins from Anemarrhena asphodeloides Bunge","volume":"34","author":"Hong YF","year":"1999","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_7_1","unstructured":"HuangCG WangXLandZhangZQ. 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