{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:39:55Z","timestamp":1780551595540,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T00:00:00Z","timestamp":1216771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new liquid chromatography\u2013mass spectrometry (LC\u2013MS) method has been developed and validated for the simultaneous determination of eight quinolone antibacterials for veterinary use in processed bovine milk samples. The quinolones studied included marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid and flumequine. Also, a new sample\u2010treatment procedure was used for extraction and preconcentration of these compounds. It involved defatting by centrifugation, protein precipitation by adding a mixture of glacial acetic acid\u2013acetonitrile and removing acetonitrile with dichloromethane; finally, the acidified aqueous layer was evaporated to dryness in a speed vac system, resuspended in the mobile phase and filtered prior to LC injection. The mobile phase was composed of a formic acid aqueous solution 0.1% (v\/v) and acetonitrile, with an initial composition of water\u2013acetonitrile 95: 5 (v\/v) and using linear gradient elution. Norfloxacin was used as internal standard. The limits of quantification found (2\u20137 ng g<jats:sup>\u22121<\/jats:sup>) were in all cases lower than the maximum residue limits tolerated by the European Union for these compounds in milk. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1041","type":"journal-article","created":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T09:23:14Z","timestamp":1216804994000},"page":"1186-1193","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography\u2013mass 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