{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:19:52Z","timestamp":1791829192359,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T00:00:00Z","timestamp":1216771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:sc>dl<\/jats:sc>\u2010Penicillamine has been resolved into its enantiomers by normal\u2010phase TLC using <jats:sc>l<\/jats:sc>\u2010tartaric acid as chiral impregnating reagent as well as chiral mobile phase additive, while (<jats:italic>R<\/jats:italic>)\u2010mandelic acid has been found to be successful as a chiral impregnating reagent. The solvent system acetonitrile\u2013methanol\u2013water (5:1:1, v\/v) was found to be successful when <jats:sc>l<\/jats:sc>\u2010tartaric acid was used as impregnating agent while the solvent combination acetonitrile\u2013methanol\u2013(0.5% <jats:sc>l<\/jats:sc>\u2010tartaric acid in water, pH 5)\u2013glacial acetic acid (7:1:1.1:0.7, v\/v) was successful as mobile phase as it contained <jats:sc>l<\/jats:sc>\u2010tartaric acid as the chiral additive. (<jats:italic>R<\/jats:italic>)\u2010mandelic acid was successful as chiral impregnating reagent with ethyl acetate\u2013methanol\u2013water (3:1:1, v\/v), as the mobile phase. The effects of concentration of chiral selectors, temperature and pH were examined on enantiomeric resolution. The spots were detected with iodine vapors and the detection limits were found to be 0.12 \u00b5g for each enantiomer of penicillamine with <jats:sc>l<\/jats:sc>\u2010tartaric acid, under both the conditions, and 0.11 \u00b5g with (<jats:italic>R<\/jats:italic>)\u2010mandelic acid. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1052","type":"journal-article","created":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T10:01:12Z","timestamp":1216807272000},"page":"1237-1242","source":"Crossref","is-referenced-by-count":29,"title":["Direct enantiomeric TLC resolution of <scp>dl<\/scp>\u2010penicillamine using (<i>R<\/i>)\u2010mandelic acid and<scp> l\u2010<\/scp>tartaric acid as chiral impregnating reagents and as chiral mobile phase additive"],"prefix":"10.1002","volume":"22","author":[{"given":"Ravi","family":"Bhushan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charu","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)94292-X"},{"key":"e_1_2_1_3_1","first-page":"373","volume-title":"Handbook of TLC","author":"Bhushan R","year":"2003"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1025"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.55"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.854"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)93517-4"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.08.132"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1998)10:5<434::AID-CHIR10>3.0.CO;2-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.07.003"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/a800712h"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92382-C"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100100463"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01772356"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100101507"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.19863190618"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD001073"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1052","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:20:30Z","timestamp":1760037630000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,23]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1002\/bmc.1052"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1052","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,23]]}}}