{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T10:17:16Z","timestamp":1771064236769,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,9,30]],"date-time":"2008-09-30T00:00:00Z","timestamp":1222732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel assay for the determination of <jats:sc>l<\/jats:sc>\u2010asparaginase activity in human plasma is described that is based on the HPLC quantitation of <jats:sc>l<\/jats:sc>\u2010aspartic acid produced during enzyme incubation. Methods for monitoring <jats:sc>l<\/jats:sc>\u2010asparagine depletion are also described. Chromatography of <jats:sc>l<\/jats:sc>\u2010aspartic acid, <jats:sc>l<\/jats:sc>\u2010asparagine and <jats:sc>l<\/jats:sc>\u2010homoserine (the internal standard) involved derivatization with <jats:italic>o<\/jats:italic>\u2010pthaldialdehyde, then separation from other amino acids on a Phenomenex Luna C<jats:sub>18<\/jats:sub> column using a 1\u00a0mL\/min flow rate and a mobile phase consisting of di\u2010potassium hydrogen orthophosphate propionate buffer, pH 6, with 10% methanol and 10% acetonitrile. Fluoresence detection was at excitation\/emission wavelengths of 357\/455 nm. Under these conditions <jats:sc>l<\/jats:sc>\u2010aspartic acid, <jats:sc>l<\/jats:sc>\u2010asparagine and <jats:sc>l<\/jats:sc>\u2010homoserine had retention times of 3.5, 9.8 and 17.7\u00a0min, respectively. The <jats:sc>l<\/jats:sc>\u2010asparaginase assay was linear from 0.1 to 10\u00a0U\/mL activity and interday precision and accuracy were less than 13%. The limit of quantitation was approximately 0.03\u00a0U\/mL. The assay utility was established in 12 children who received <jats:italic>E<\/jats:italic>.<jats:italic> coli<\/jats:italic> <jats:sc>l<\/jats:sc>\u2010asparaginase as treatment for acute lymphoblastic leukaemia. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1096","type":"journal-article","created":{"date-parts":[[2008,9,30]],"date-time":"2008-09-30T05:37:13Z","timestamp":1222753033000},"page":"152-159","source":"Crossref","is-referenced-by-count":24,"title":["An isocratic fluorescence HPLC assay for the monitoring of <scp>l<\/scp>\u2010asparaginase activity and <scp> l<\/scp>\u2010asparagine depletion in children receiving <i> E<\/i>.<i> coli<\/i><scp>l<\/scp>\u2010asparaginase for the treatment of acute lymphoblastic leukaemia"],"prefix":"10.1002","volume":"23","author":[{"given":"Christa E.","family":"Nath","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano","family":"Dallapozza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam E.","family":"Eslick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Misra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Carr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Earl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,30]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"4363","article-title":"In vitro and in vivo killing of acute lymphoblastic leukaemia cells by l\u2010asparaginase","volume":"49","author":"Asselin BH","year":"1989","journal-title":"Cancer Research"},{"key":"e_1_2_1_3_1","first-page":"6568","article-title":"Measurement of serum l\u2010asparagine in the presence of l\u2010asparaginase requires the presence of an l\u2010asparaginase 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