{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:10:31Z","timestamp":1790935831541,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2001,10,19]],"date-time":"2001-10-19T00:00:00Z","timestamp":1003449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A simple, rapid and sensitive fluorescence high performance liquid chromatographic method was developed to determine propranolol concentration in the small volume of rat plasma without the solvent extraction step using pronethanol as the internal standard. The analysis was accomplished using a 5\u2005\u00b5m CAPCELL PAK analytical cyano column at room temperature and a mobile phase consisted of 1% aqueous acetic acid containing 0.2% triethylamine and acetonitrile (65:35, v\/v; pH 3.8). The flow\u2010rate was kept at 0.5\u2005mL\/min and column effluent was monitored with a fluorescence detector at an excitation wavelength of 230\u2005nm and an emission wavelength of 340\u2005nm. Retention times for pronethalol and propranolol were 8.5\u2005min and 10.5\u2005min, respectively. Linear regressions for the standard curves were linear in the range 2\u2013800\u2005ng\/mL, giving correlation coefficients above 0.998. The detection limit was 1.34\u2005ng\/mL. No analytical interference was observed from endogenous components in rat plasma. This simple and sensitive assay method was feasibly applied to the pharmacokinetic study of propranolol after intravenous administration of 2\u2005mg\/kg of propranolol to normal and carbon tetrachloride\u2010induced liver cirrhotic rats. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.110","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T11:25:40Z","timestamp":1031657140000},"page":"539-545","source":"Crossref","is-referenced-by-count":18,"title":["Determination of propranolol concentration in small volume of rat plasma by HPLC with fluorometric detection"],"prefix":"10.1002","volume":"15","author":[{"given":"H.K.","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.H.","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.S.","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.S.","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.H.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2710.1991.tb00289.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/cpt1979262173","article-title":"Radioreceptor assay for propranolol","volume":"26","author":"Bilezkian JP","year":"1979","journal-title":"Clinical Pharmacology and Therapy"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1965.tb01782.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.52.2.313"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)91714-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600700916"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00260a028"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600670931"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)84603-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80488-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90261-X"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015882607690"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600661040"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600750121"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80061-0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81695-3"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/20.3.126"},{"key":"e_1_2_1_19_1","first-page":"515","article-title":"Rapid determination of propranolol and 4\u2010hydroxypropranolol in plasma by high\u2010performance liquid chromatography","volume":"10","author":"Mason WD","year":"1977","journal-title":"Analytical Letters"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81372-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(78)90442-4"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(95)01575-2"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81315-8"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/cpt1970111112","article-title":"Plasma propranolol levels in adults with observations in four children","volume":"11","author":"Shand DG","year":"1970","journal-title":"Clinical Pharmacology Therapy"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-198802000-00018"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt1977226853"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600631212"},{"key":"e_1_2_1_28_1","first-page":"991","article-title":"Quantitative GC\u2010MS of propranolol. A key to an improved understanding of the relationship between plasma concentrations and dose","volume":"24","author":"Walle T","year":"1978","journal-title":"Clinical Chemistry"},{"key":"e_1_2_1_29_1","first-page":"235","volume-title":"The Pharmacological Basis of Therapeutics","author":"Weiner N","year":"1996"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1093\/clinchem\/22.6.832","article-title":"Tabulation of therapeutic, toxic, and lethal concentrations of drugs and chemicals in blood","volume":"22","author":"Winek CL","year":"1976","journal-title":"Clinical Chemistry"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb1978.4.879"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:59:11Z","timestamp":1760043551000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,19]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1002\/bmc.110"],"URL":"https:\/\/doi.org\/10.1002\/bmc.110","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,19]]}}}