{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T09:12:08Z","timestamp":1793351528428,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":5939,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A gas chromatographic\u2013mass spectrometric (GC\/MS) method for analysis of \u0394<jats:sup>1<\/jats:sup>\u2010tetrahydrocannabinol (\u0394<jats:sup>1<\/jats:sup>\u2010THC) in human fat samples is described. The fat sample, obtained from heavy marihuana users 1 week before and 4 weeks after smoking, is homogenized in hexane + 2\u2010propanol, centrifuged, and the supernatant mixed with Lipidex 5000. The solvent is evaporated and the dried gel is packed in a glass column. \u0394<jats:sup>1<\/jats:sup> \u2010THC is eluted from the column with methanol + water + acetic acid, diluted with water and the eluent is passed through a bed of Octadecylsilanebonded silica. After washing and drying, the retained \u0394<jats:sup>1<\/jats:sup>\u2010THC is eluted with hexane, derivatized with N\u2010methyl\u2010N\u2010(<jats:italic>t<\/jats:italic>\u2010butyl\u2010dimethysilyl)trifluoroacetamide (MTBSTFA) and finally purified by HPLC on an Octadecyl SI 100 column in methanol. The amount of \u0394<jats:sup>1<\/jats:sup>\u2010THC is determined by GC\/MS, using selected ion monitoring, and a deuterated internal standard. The recovery of \u0394<jats:sup>1<\/jats:sup>\u2010THC is about 80%, and the concentration of \u0394<jats:sup>1<\/jats:sup>\u2010THC in the fat samples analysed ranged between 0.4 and 193 ng\/g wet tissue.<\/jats:p>","DOI":"10.1002\/bmc.1130030109","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:03:04Z","timestamp":1117281784000},"page":"35-38","source":"Crossref","is-referenced-by-count":113,"title":["Determination of \u0394<sup>1<\/sup>\u2010tetrahydrocannabinol in human fat biopsies from marihuana users by gas chromatography\u2013mass spectrometry"],"prefix":"10.1002","volume":"3","author":[{"given":"Eva","family":"Johansson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koidu","family":"Nor\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Sj\u00f6vall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus M.","family":"Halldin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"391","volume":"8","author":"Agurell S.","year":"1971","journal-title":"Acta Pharm. 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