{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:40:51Z","timestamp":1760046051091,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":5331,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A convenient high performance liquid chromatographic method for the determination of methylxanthines in biological samples is described. Separation was achieved by reversed phase chromatography using a mobile phase consisting of tetrahydrofuran + methanol + 0.01M potassium dihydrogen phosphate, pH 3.5 (1:20:79, v\/v\/v), on a 7 \u03bcm C<jats:sub>18<\/jats:sub> column and a C<jats:sub>18<\/jats:sub> Lichrosorb precolumn at a flow rate of 0.8 mL\/min. Levels varying from 0.25 \u2013 16 mg\/L could be detected by UV at 280 nm. In this range, standard curves were established for 4 methylxanthines: theobromine, paraxanthine, theophylline and caffeine in 4 media: mobile phase, serum, gastric and pancreatic juices, and were found to be linear (<jats:italic>r<\/jats:italic> \u2265 0.9975). Overall characteristics of the method were determined as: percent recovery (89.54%), accuracy (\u226599.4%) and reproducibility (\u226595%). Retention times ranged from 4.21 \u00b1 0.01 (1\u2010methyluric acid) to 10.8 \u00b1 0.03 min (caffeine). Animal experiments (5 and 10 mg\/kg boluses) were used to determine caffeine half life in dog's blood (310 \u00b1 46 and 453 \u00b1 59 min, respectively) and its secretion into pentagastrin stimulated gastric juice (mean concentrations 2.51 and 6.04 mg\/L; mean outputs 351 and 1206 \u03bcg\/2.25 h; both statistically different at <jats:italic>p<\/jats:italic> &lt; 0.001 level).<\/jats:p>","DOI":"10.1002\/bmc.1130040508","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:14:04Z","timestamp":1117282444000},"page":"209-213","source":"Crossref","is-referenced-by-count":7,"title":["High performance liquid chromatographic determination of methylxanthines in canine serum, gastric and pancreatic juices"],"prefix":"10.1002","volume":"4","author":[{"given":"Philippe","family":"Casoli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"V\u00e9rine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"378","volume":"7","author":"Aldridge A.","year":"1979","journal-title":"Drug. 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