{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:28:33Z","timestamp":1761596913261,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":5209,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Racemic phenylpropanolamine was resolved on a high performance liquid chromatographic (HPLC) chiral stationary phase (CSP) as the 3,5\u2010dinitrophenyl ureide derivative. The CSP was prepared by a simple <jats:italic>in situ<\/jats:italic> procedure in which (<jats:italic>R<\/jats:italic>)\u2010(1\u2010naphthyl)ethyl isocyanate was bound to aminopropyl silanized silica through a urea linkage. The enantiomeric ureides were prepared by a room\u2010temperature, 60\u2010second procedure, accomplishing simultaneous extraction and derivatization and utilizing achiral 3,5\u2010dinitrophenyl isocyanate as reagent. Baseline resolution was readily achieved under normal phase conditions, with a separation factor (\u03b1) of 1.16 and a resolution factor (<jats:italic>R<\/jats:italic><jats:sub>s<\/jats:sub>) of 2.2. Elution was complete within 10 min. A limit of detection, by UV at 235 nm, of 250 pg per isomer was established. Feasibility of the procedure for plasma determinations was demonstrated by assay of samples from a canine subject.<\/jats:p>","DOI":"10.1002\/bmc.1130050110","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T18:57:06Z","timestamp":1117220226000},"page":"43-46","source":"Crossref","is-referenced-by-count":6,"title":["Enantiomeric analysis of phenylpropanolamine in plasma via resolution of dinitrophenylurea derivatives on a high performance liquid chromatographic chiral stationary phase"],"prefix":"10.1002","volume":"5","author":[{"given":"Thomas D.","family":"Doyle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte A.","family":"Brunner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Vick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)94529-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600730936"},{"volume-title":"Chiral Liquid Chromatography","year":"1989","author":"Doyle T. 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Chromatogr."}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1130050110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1130050110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:21:26Z","timestamp":1760044886000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1130050110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/bmc.1130050110"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1130050110","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}