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Under the optimized conditions, only the sulphonamide drugs with an unsubstituted sulphonamide group were retained on the CA precolumn and separated on a reversed\u2010phase analytical column. Of these, the retention of hydrochlorothiazide (HCT), chlorothiazide, acetazolamide, furosemide (FS) and chlorthalidone was almost quantitative. The peak area of HCT was proportional to the concentration in the range of 1\u2013100 nmol\/mL with relative standard deviations of 3.7% (5 nmol\/mL) and 0.7% (100 nmol\/mL).<\/jats:p><jats:p>This CS system was applied to urine and plasma samples spiked with HCT and FS. Endogenous components of these were effectively removed, and HCT and FS were selectively retained on the CA precolumn. Almost quantitative recoveries and reproducibility were obtained.<\/jats:p>","DOI":"10.1002\/bmc.1130080408","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:33:23Z","timestamp":1117283603000},"page":"184-188","source":"Crossref","is-referenced-by-count":3,"title":["Carbonic anhydrase\u2010immobilized precolumn for selective on\u2010line sample pretreatment in high\u2010performance liquid chromatographic determination of certain sulphonamide drugs"],"prefix":"10.1002","volume":"8","author":[{"given":"Takafumi","family":"Ohta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ittetsu","family":"Takamiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoji","family":"Takitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99681-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)85547-7"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1016\/S0021-9258(18)99413-5","volume":"242","author":"Colemann J. 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