{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:56:49Z","timestamp":1760061409284,"version":"build-2065373602"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":3932,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An analytical method has been developed with which to measure the microsomal enzyme activities responsible for oxidative theophylline metabolism. Three metabolites: 3\u2010Methylxanthine (3\u2010MX); 1\u2010methylxanthine (1\u2010MX); 1,3\u2010dimethyluric acid (1,3\u2010DMU), with acetaminophen as an internal standard (IS), were separated by solid phase extraction using a Sep\u2010Pak C<jats:sub>18<\/jats:sub> cartridge, followed by high performance liquid chromatography on a reversed\u2010phase column with isocratic elution using 25 m<jats:sc>M<\/jats:sc> acetate buffer containing 4% acetonitrile and 2.5 m<jats:sc>M<\/jats:sc> tetra\u2010<jats:italic>n<\/jats:italic>\u2010butylammonium hydrogen sulphate (pH 5.25) as the mobile phase. The analytes were clearly resolved and no interference with foreign peaks was observed. A linear relationship was obtained for the metabolites over the concentration range of 0.5\u20135.0 \u03bcg\/mL, and their analytical recovery was almost 100%. This method can be used to assess drug interactions involving alterations in the biotransformation of theophylline.<\/jats:p>","DOI":"10.1002\/bmc.1130080409","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T12:33:23Z","timestamp":1117283603000},"page":"189-192","source":"Crossref","is-referenced-by-count":6,"title":["Measurement of theophylline metabolites produced by reaction with hepatic microsome by high performance liquid chromatography following solid phase extraction"],"prefix":"10.1002","volume":"8","author":[{"given":"Hiroki","family":"Konishi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Yamaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/S0090-9556(25)00269-7","volume":"8","author":"Betlach C. 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